A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion

Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to conferencePaperpeer-review

Abstract

A β-(1→3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This paper describes our attempt to apply the SPG complex to deliver CpG DNA to endosome in order to enhance cytokine secretion. To increase cellular up-take, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG shows essentially no-cytotoxicity. When CpG DMA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose, and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA. The paper will include a recent result for in-vivo assay carried out by Profs. Akira and Ishii at Osaka Univ.

Original languageEnglish
Pages5110-5111
Number of pages2
Publication statusPublished - Dec 1 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: Sep 20 2005Sep 22 2005

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period9/20/059/22/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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