A possible maternal-effect mutant of Xenopus laevis: II. Studies of RNA synthesis in dissociated embryonic cells

Koichiro Shiokawa, Kosuke Tashiro, Norihiko Nakakura, Yuchang Fu, Yasuo Atsuchi, Sakiko Nakazato, Yoshinari Tsuzaki, Kohji Ikenishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Embryos from a female of Xenopus laevis (designated as no. 65) arrest development at gastrulation and are assumed to be ova-deficient mutant. We dissociated these embryos and studied RNA synthesis at different stages. The cells from the ova-deficient embryos reaggregated quite actively as wild-type embryo cells until the late gastrula stage. RNA synthesis was normal at the early blastula stage but greatly inhibited by the late blastula (stage 9.5) stage, when the synthesis of DNA and protein was still not inhibited appreciably. Thus, inhibition in RNA synthesis appears to be the first manifestation of the maternal defect that occurs before the gastrulation arrest.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalCell Differentiation and Development
Volume25
Issue number1
DOIs
Publication statusPublished - Sep 1988

Fingerprint

Xenopus laevis
Embryonic Structures
RNA
Blastula
Gastrulation
Ovum
Gastrula
Mothers
Maternal Inheritance
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

A possible maternal-effect mutant of Xenopus laevis : II. Studies of RNA synthesis in dissociated embryonic cells. / Shiokawa, Koichiro; Tashiro, Kosuke; Nakakura, Norihiko; Fu, Yuchang; Atsuchi, Yasuo; Nakazato, Sakiko; Tsuzaki, Yoshinari; Ikenishi, Kohji.

In: Cell Differentiation and Development, Vol. 25, No. 1, 09.1988, p. 47-55.

Research output: Contribution to journalArticle

Shiokawa, Koichiro ; Tashiro, Kosuke ; Nakakura, Norihiko ; Fu, Yuchang ; Atsuchi, Yasuo ; Nakazato, Sakiko ; Tsuzaki, Yoshinari ; Ikenishi, Kohji. / A possible maternal-effect mutant of Xenopus laevis : II. Studies of RNA synthesis in dissociated embryonic cells. In: Cell Differentiation and Development. 1988 ; Vol. 25, No. 1. pp. 47-55.
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