A Protein-bound polysaccharide, PSK, enhances tumor suppression induced by docetaxel in a gastric cancer xenograft model

Akio Yamasaki, Momoko Shoda, Hiroko Iijima, Shuntaro Nagai, Junji Wada, Hiroyuki Suzuki, Nobuhito Chikazawa, Takehiko Tasaka, Chizu Kameda, Haruo Tanaka, Ikebe Mio, Jo Ei, Norihiro Sato, Masafumi Nakamura, Fujio Serine, Takashi Morisaki, Mitsuo Katano

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Background: We have reported previously that docetaxel (TXT) induces apoptosis and nuclear factor-kappaB (NF-κB) activation, and that blockade of NF-κB activation augments TXT- induced apoptosis in human gastric cancer cells. In addition, we have also shown that a protein-bound polysaccharide PSK enhances TXT-induced apoptosis through NF-κB inhibition in human pancreatic cancer cells. Based on these observations, in the present study the possibility that PSK could enhance TXT- mediated tumor suppression was examined in vivo and in vitro. Materials and Methods: A gastric cancer xenograft model was used to examine the enhanced TXT- mediated tumor suppression by PSK in vivo. The effects of PSK on proliferation and apoptosis induced by TXT in gastric cancer cells were evaluated in vitro using a human gastric cancer cell line, MK-1. The effect of PSK on increased TXT-induced invasion was also measured. Results: PSK enhanced TXT-mediated tumor suppression in vivo. Immunohistochemical analyses of the tumors revealed that TXT increased NF-κB activation in the tumors and this was suppressed by PSK. In the ex vivo experimental system, PSK enhanced the growth inhibition and apoptosis induced by TXT in the MK-1 cells and reduced the increased invasive ability induced by TXT. Conclusion: PSK enhanced TXT-induced tumor suppression in a gastric cancer xenograft model.

Original languageEnglish
Pages (from-to)843-850
Number of pages8
JournalAnticancer research
Volume29
Issue number3
Publication statusPublished - Mar 2009

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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