A protein friction model of the actin sliding movement generated by myosin in mixtures of MgATP and MgGTP in vitro

Yasuhiro Imafuku, Yumiko Emoto, Katsuhisa Tawada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The sliding movement of an actin filament generated by myosin heads with MgGTP bound is much slower than that by those with MgATP bound. Nonetheless, there is a report that the actin sliding velocity at low (11-21 μM) MgATP concentrations is increased by the addition of MgGTP in a range of 1-3 mM, although the actin sliding velocity at these MgATP concentrations is larger than the maximum sliding velocity attained in the presence of MgGTP alone. The convex rise in the velocity was called 'mutual sensitization of MgATP and MgGTP' in the report. Here we propose a theoretical model to account for the mutual sensitization of MgATP and MgGTP. The model is an extension of a protein friction model, accommodating the presence of two different substrates and assuming the presence of motile and non-motile myosins. This new model is in accord with the characteristics of the actin/myosin sliding movement experimentally observed in mixtures of MgATP and MgGTP. Comparison of the model with the experimental results implies that the non-motile and motile myosins are those with the 'converse and correct' orientations of their heads with respect to the direction of the actin sliding movement in vitro.

Original languageEnglish
Pages (from-to)359-370
Number of pages12
JournalJournal of Theoretical Biology
Volume199
Issue number4
DOIs
Publication statusPublished - Aug 21 1999

Fingerprint

Myosin
Friction
Actin
Myosins
myosin
friction
actin
Actins
Adenosine Triphosphate
Proteins
Protein
proteins
Theoretical Models
Model
Filament
microfilaments
Converse
Theoretical Model
Actin Cytoskeleton
Substrate

All Science Journal Classification (ASJC) codes

  • Statistics and Probability
  • Modelling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

A protein friction model of the actin sliding movement generated by myosin in mixtures of MgATP and MgGTP in vitro. / Imafuku, Yasuhiro; Emoto, Yumiko; Tawada, Katsuhisa.

In: Journal of Theoretical Biology, Vol. 199, No. 4, 21.08.1999, p. 359-370.

Research output: Contribution to journalArticle

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