A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

Shujiro Shiosaki, Takanobu Nobori, Takeshi Mori, Riki Toita, Yuta Nakamura, Chan Woo Kim, Tatsuhiro Yamamoto, Takuro Niidome, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.

    Original languageEnglish
    Pages (from-to)5592-5594
    Number of pages3
    JournalChemical Communications
    Volume49
    Issue number49
    DOIs
    Publication statusPublished - May 23 2013

    All Science Journal Classification (ASJC) codes

    • Catalysis
    • Electronic, Optical and Magnetic Materials
    • Ceramics and Composites
    • Chemistry(all)
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Materials Chemistry

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