A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

Shujiro Shiosaki, Takanobu Nobori, Takeshi Mori, Riki Toita, Yuta Nakamura, Chan Woo Kim, Tatsuhiro Yamamoto, Takuro Niidome, Yoshiki Katayama

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28 Citations (Scopus)

Abstract

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.

Original languageEnglish
Pages (from-to)5592-5594
Number of pages3
JournalChemical Communications
Volume49
Issue number49
DOIs
Publication statusPublished - May 23 2013

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All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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