A proteinase inhibitor from egg yolk of hen is an ovoinhibitor analog

Yasushi Sugimoto, Takahiro Kusakabe, Sumiharu Nagaoka, Takashi Nirasawa, Kazue Tatsuguchi, Makoto Fujii, Takayoshi Aoki, Katsumi Koga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A proteinase inhibitor, tentatively termed vitelloinhibitor, was purified from yolk of hen's ovarian follicles. It resembled egg-white ovoinhibitor not only in inhibitory spectrum (active for bovine trypsin and bovine chymotrypsin) but also in thermal stability, pH stability, antiserum reactivity and amino-acid composition. However, vitelloinhibitor had different molecular weight from that of ovoinhibitor. An α2-proteinase inhibitor preparation, isolated from laying hen's serum in the present study, was found to exhibit two bands, and the larger one of the latter corresponded to vitelloinhibitor in molecular weight. The partial N-terminal amino-acid sequence of vitelloinhibitor was the same as those of the two components of serum inhibitor and all three agreed with that of ovoinhibitor. Vitelloinhibitor is likely to be an ovoinhibitor analog derived from a serum precursor, which might be the larger component of α2-proteinase inhibitor.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalBiochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
Volume1295
Issue number1
DOIs
Publication statusPublished - Jun 7 1996

Fingerprint

Egg Yolk
Peptide Hydrolases
Molecular Weight
Serum
Molecular weight
Amino Acids
Egg White
Ovarian Follicle
Chymotrypsin
Trypsin
Immune Sera
Amino Acid Sequence
Thermodynamic stability
Hot Temperature
ovoinhibitor
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

A proteinase inhibitor from egg yolk of hen is an ovoinhibitor analog. / Sugimoto, Yasushi; Kusakabe, Takahiro; Nagaoka, Sumiharu; Nirasawa, Takashi; Tatsuguchi, Kazue; Fujii, Makoto; Aoki, Takayoshi; Koga, Katsumi.

In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, Vol. 1295, No. 1, 07.06.1996, p. 96-102.

Research output: Contribution to journalArticle

Sugimoto, Yasushi ; Kusakabe, Takahiro ; Nagaoka, Sumiharu ; Nirasawa, Takashi ; Tatsuguchi, Kazue ; Fujii, Makoto ; Aoki, Takayoshi ; Koga, Katsumi. / A proteinase inhibitor from egg yolk of hen is an ovoinhibitor analog. In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology. 1996 ; Vol. 1295, No. 1. pp. 96-102.
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