A pulsed-field gel electrophoresis typing scheme for Vibrio parahaemolyticus isolates from fifteen countries

Hin chung Wong, Shu Hui Liu, Chien Shun Chiou, Mitsuaki Nishibuchi, Bok Kwon Lee, Orasa Suthienkul, Gopinath Balakrish Nair, Charles A. Kaysner, Hatsumi Taniguchi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is an important foodborne pathogen in Taiwan and many other maritime Asian countries where seafood is frequently consumed. A total of 535 strains of V. parahaemolyticus were recovered mostly (97%) from clinical samples obtained in Taiwan or in 14 other countries. These strains were typed by pulsed-field gel electrophoresis following SfiI digestion and a typing scheme was generated. The 115 different patterns identified were grouped into 13 types with dissimilarity values less than 15, plus 16 miscellaneous patterns not grouped into any of the types. Types I, A, D and J contained the most patterns, with the numbers of patterns being 17, 13, 12, and 11, respectively. However, types I, B, D, A, H and C contained the most strains, with the numbers of strains being 204, 73, 71, 54, 29 and 25, respectively. Type I consisted exclusively of the pandemic O3:K6 strains and genetically closely related strains. This PFGE typing scheme for V. parahaemolyticus could be used for the characterization of pathogenic isolates.

Original languageEnglish
Pages (from-to)280-287
Number of pages8
JournalInternational Journal of Food Microbiology
Volume114
Issue number3
DOIs
Publication statusPublished - Mar 20 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

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