A putative type III secretion system effector encoded by the MA20_12780 gene in Bradyrhizobium japonicum Is-34 causes incompatibility with Rj4 genotype soybeans

Hirohito Tsurumaru, Syougo Hashimoto, Kouhei Okizaki, Yu Kanesaki, Hirofumi Yoshikawa, Takeo Yamakawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The nodulation of Bradyrhizobium japonicum Is-34 is restricted by Rj4 genotype soybeans (Glycine max). To identify the genes responsible for this incompatibility, Tn5 mutants of B. japonicum Is-34 that were able to overcome this nodulation restriction were obtained. Analysis of the Tn5 mutants revealed that Tn5 was inserted into a region containing the MA20_12780 gene. In addition, direct disruption of this gene using marker exchange overcame the nodulation restriction by Rj4 genotype soybeans. The MA20_12780 gene has a tts box motif in its upstream region, indicating a possibility that this gene encodes a type III secretion system (T3SS) effector protein. Bioinformatic characterization revealed that the MA20_12780 protein contains the small ubiquitin-like modifier (SUMO) protease domain of the C48 peptidase (ubiquitin-like protease 1 [Ulp1]) family. The results of the present study indicate that a putative T3SS effector encoded by the MA20_12780 gene causes the incompatibility with Rj4 genotype soybeans, and they suggest the possibility that the nodulation restriction of B. japonicum Is-34 may be due to Rj4 genotype soybeans recognizing the putative T3SS effector (MA20_12780 protein) as a virulence factor.

Original languageEnglish
Pages (from-to)5812-5819
Number of pages8
JournalApplied and environmental microbiology
Volume81
Issue number17
DOIs
Publication statusPublished - Jan 1 2015

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Bradyrhizobium
type III secretion system
Bradyrhizobium japonicum
incompatibility
Soybeans
secretion
soybean
genotype
nodulation
Genotype
soybeans
gene
Genes
ubiquitin
Peptide Hydrolases
genes
Ubiquitin
proteinases
protein
mutants

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

A putative type III secretion system effector encoded by the MA20_12780 gene in Bradyrhizobium japonicum Is-34 causes incompatibility with Rj4 genotype soybeans. / Tsurumaru, Hirohito; Hashimoto, Syougo; Okizaki, Kouhei; Kanesaki, Yu; Yoshikawa, Hirofumi; Yamakawa, Takeo.

In: Applied and environmental microbiology, Vol. 81, No. 17, 01.01.2015, p. 5812-5819.

Research output: Contribution to journalArticle

Tsurumaru, Hirohito ; Hashimoto, Syougo ; Okizaki, Kouhei ; Kanesaki, Yu ; Yoshikawa, Hirofumi ; Yamakawa, Takeo. / A putative type III secretion system effector encoded by the MA20_12780 gene in Bradyrhizobium japonicum Is-34 causes incompatibility with Rj4 genotype soybeans. In: Applied and environmental microbiology. 2015 ; Vol. 81, No. 17. pp. 5812-5819.
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