A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo, Hiromu Ikeda, Junpei Kamimoto, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.

    Original languageEnglish
    Pages (from-to)658-660
    Number of pages3
    JournalChemistry Letters
    Volume43
    Issue number5
    DOIs
    Publication statusPublished - 2014

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)

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