A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS

Yuki Otsubo, Hiromu Ikeda, Junpei Kamimoto, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

Abstract

A rapid and quantitative measurement of protein kinase activity based on MALDI-TOF-MS was developed. Here, biotinylated substrate peptides with stable isotope labeling were used to enable the rapid and specific enrichment of the peptides. Protein kinase activities in two samples can be directly compared utlizing the mass-unit difference of the subsrate peptides with different isotope labels. We successfully evaluated the effect of an inhibitory drug on the cellular protein kinase acitivity.

Original languageEnglish
Pages (from-to)658-660
Number of pages3
JournalChemistry Letters
Volume43
Issue number5
DOIs
Publication statusPublished - Jan 1 2014

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Protein Kinases
Isotopes
Peptides
Labeling
Labels
Substrates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS. / Otsubo, Yuki; Ikeda, Hiromu; Kamimoto, Junpei; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.

In: Chemistry Letters, Vol. 43, No. 5, 01.01.2014, p. 658-660.

Research output: Contribution to journalArticle

Otsubo, Yuki ; Ikeda, Hiromu ; Kamimoto, Junpei ; Niidome, Takuro ; Mori, Takeshi ; Katayama, Yoshiki. / A rapid and quantitative detection of cellular protein kinase activity based on MALDI-TOF-MS. In: Chemistry Letters. 2014 ; Vol. 43, No. 5. pp. 658-660.
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