A rapid one-step immunochromatographic assay for the detection of asiaticoside

Boonchoo Sritularak, Thaweesak Juengwatanatrakul, Waraporn Putalun, Hiroyuki Tanaka, Satoshi Morimoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Asiaticoside has been identified as the most active compound in Centella asiatica. In order to screen a large number of plant samples for the presence of asiaticoside, a rapid and simple technique is required that utilizes small quantities for test samples. In this study, an immunochromatographic strip test has been developed for the detection of asiaticoside in plant samples that uses a monoclonal antibody against asiaticoside. The limit of detection for the strip test was 12.5 μg/ml. Immunoassay using monoclonal antibodies could be useful for the determination of small quantities of asiaticoside in plant extracts.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalJournal of Natural Medicines
Volume66
Issue number2
DOIs
Publication statusPublished - Apr 1 2012

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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