A rare point mutation in the Ras oncogene in hepatocellular carcinoma

Akinobu Taketomi, Ken Shirabe, Jun Muto, Shohei Yoshiya, Takashi Motomura, Yohei Mano, Tohru Ikegami, Tomoharu Yoshizumi, Kenji Sugio, Yoshihiko Maehara

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14 Citations (Scopus)

Abstract

Purpose: The Ras gene is one of the oncogenes most frequently detected in human cancers, and codes for three proteins (K-, N-, and H-Ras). The aim of this study was to examine the mutations in codons 12, 13 and 61 of the three Ras genes in cases of human hepatocellular carcinoma (HCC). Methods: Paired samples of HCC and corresponding non-malignant liver tissue were collected from 61 patients who underwent hepatectomy. A dot-blot analysis was used to analyze the products of the polymerase chain reaction (PCR) amplification of codons 12, 13, and 61 of K-, N- and H-Ras for mutations. Results: Only one mutation (K-Ras codon 13; Gly to Asp) was detected among the 61 patients. Interestingly, this patient had a medical history of surgery for both gastric cancer and right lung cancer. No mutations were found in codons 12 and 61 of K-Ras or codons 12, 13 and 61 of the N-Ras and H-Ras genes in any of the HCCs or corresponding non-malignant tissues. Conclusions: These findings indicated that the activation of Ras proto-oncogenes by mutations in codons 12, 13, and 61 does not play a major role in hepatocellular carcinogenesis.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalSurgery today
Volume43
Issue number3
DOIs
Publication statusPublished - Mar 1 2013

All Science Journal Classification (ASJC) codes

  • Surgery

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    Taketomi, A., Shirabe, K., Muto, J., Yoshiya, S., Motomura, T., Mano, Y., Ikegami, T., Yoshizumi, T., Sugio, K., & Maehara, Y. (2013). A rare point mutation in the Ras oncogene in hepatocellular carcinoma. Surgery today, 43(3), 289-292. https://doi.org/10.1007/s00595-012-0462-8