A rat monoclonal antibody against the chromatin remodeling factor CHD5

Saori Yoshimura, Tomohiko Yoshimi, Yasuyuki Ohkawa, Masayuki Azuma, Taro Tachibana

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a hybridoma cell line for producing a monoclonal antibody against CHD5 by the rat medial iliac lymph node method. Immunoblotting analyses indicated that this antibody, MAb 5A10, specifically recognizes endogenous CHD5. In immunostaining using the antibody, a nuclear staining pattern was observed. The monoclonal antibody will be useful in immunoblotting and immunolocalization experiments in a variety of cells and tissues, as well as in further studies of the biological function and cellular dynamics of this protein.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalHybridoma
Volume29
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

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Chromatin Assembly and Disassembly
DNA-Binding Proteins
Monoclonal Antibodies
Immunoblotting
Antibodies
Hybridomas
Neoplasms
Proteins
Lymph Nodes
Staining and Labeling
Cell Line

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

A rat monoclonal antibody against the chromatin remodeling factor CHD5. / Yoshimura, Saori; Yoshimi, Tomohiko; Ohkawa, Yasuyuki; Azuma, Masayuki; Tachibana, Taro.

In: Hybridoma, Vol. 29, No. 1, 01.02.2010, p. 63-66.

Research output: Contribution to journalArticle

Yoshimura, S, Yoshimi, T, Ohkawa, Y, Azuma, M & Tachibana, T 2010, 'A rat monoclonal antibody against the chromatin remodeling factor CHD5', Hybridoma, vol. 29, no. 1, pp. 63-66. https://doi.org/10.1089/hyb.2009.0069
Yoshimura, Saori ; Yoshimi, Tomohiko ; Ohkawa, Yasuyuki ; Azuma, Masayuki ; Tachibana, Taro. / A rat monoclonal antibody against the chromatin remodeling factor CHD5. In: Hybridoma. 2010 ; Vol. 29, No. 1. pp. 63-66.
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