A reduction in the number of peripheral CD28+ CD8+ T cells in the acute phase of influenza

S. Nabeshima, Murata Masayuki, K. Kikuchi, H. Ikematsu, S. Kashiwagi, J. Hayashi

Research output: Contribution to journalArticle

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Abstract

Influenza patients show a high incidence of T lymphocytopenia in the acute phase of the illness. Since CD8+ T cells play an important role in influenza virus infection, we investigated which subset of CD8+ T cells was involved in this lymphocytopenia. CD8+ T cells from eight patients with influenza A were studied for lymphocyte count, surface marker, and intracellular IFN-γ production in the acute (days 1-3) and recovery phases (days 9-12). Total and T lymphocyte counts in the acute phase were approximately three times less than in the recovery phase; however, the CD4/8 ratio was the same in both phases. The cell count reduction in the acute phase was attributed predominantly to the CD28+ CD8+ subset, compared with the CD28- CD8+ subset. The memory/activation marker CD45RO on the CD8+ T cells was assessed. The CD28+ CD45RO- subset, a naive phenotype, was reduced significantly in number in the acute phase compared with the recovery phase. The CD28+ CD45RO+ subset, a memory phenotype, was also reduced in the acute phase, but the reduction was not statistically significant. Intracellular IFN-γ in the CD8+ subset after mitogenic stimulation was measured by flow cytometry; the percentage of CD28+ IFN-γ-/CD8+ subset in the acute phase was significantly less than in the recovery phase. These results indicated that the predominant reduction of peripheral CD8+ T cells in the acute phase of influenza was from naive-type lymphocytes, suggesting that these quantitative and qualitative changes of CD8+ T cells in influenza are important for understanding the immunological pathogenesis.

Original languageEnglish
Pages (from-to)339-346
Number of pages8
JournalClinical and Experimental Immunology
Volume128
Issue number2
DOIs
Publication statusPublished - May 30 2002

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Human Influenza
T-Lymphocytes
Lymphocyte Count
Phenotype
Lymphopenia
T-Lymphocyte Subsets
Virus Diseases
Orthomyxoviridae
Flow Cytometry
Cell Count
Lymphocytes
Incidence

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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A reduction in the number of peripheral CD28+ CD8+ T cells in the acute phase of influenza. / Nabeshima, S.; Masayuki, Murata; Kikuchi, K.; Ikematsu, H.; Kashiwagi, S.; Hayashi, J.

In: Clinical and Experimental Immunology, Vol. 128, No. 2, 30.05.2002, p. 339-346.

Research output: Contribution to journalArticle

Nabeshima, S. ; Masayuki, Murata ; Kikuchi, K. ; Ikematsu, H. ; Kashiwagi, S. ; Hayashi, J. / A reduction in the number of peripheral CD28+ CD8+ T cells in the acute phase of influenza. In: Clinical and Experimental Immunology. 2002 ; Vol. 128, No. 2. pp. 339-346.
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