A replicase clamp-binding dynamin-like protein promotes colocalization of nascent DNA strands and equipartitioning of chromosomes in E.coli

Shogo Ozaki, Yusaku Matsuda, Kenji Keyamura, Hironori Kawakami, Yasunori Noguchi, Kazutoshi Kasho, Komomo Nagata, Tamami Masuda, Yukari Sakiyama, Tsutomu Katayama

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In Escherichia coli, bidirectional chromosomal replication is accompanied by the colocalization of sister replication forks. However, the biological significance of this mechanism and the key factors involved are still largely unknown. In this study, we found that a protein, termed CrfC, helps sustain the colocalization of nascent DNA regions of sister replisomes and promote chromosome equipartitioning. CrfC formed homomultimers that bound to multiple molecules of the clamp, a replisome subunit that encircles DNA, and colocalized with nascent DNA regions in a clamp-binding-dependent manner in living cells. CrfC is a dynamin homolog; however, it lacks the typical membrane-binding moiety and instead possesses a clamp-binding motif. Given that clamps remain bound to DNA after Okazaki fragment synthesis, we suggest that CrfC sustains the colocalization of sister replication forks in a unique manner by linking together the clamp-loaded nascent DNA strands, thereby laying the basis for subsequent chromosome equipartitioning

Original languageEnglish
Pages (from-to)985-995
Number of pages11
JournalCell Reports
Volume4
Issue number5
DOIs
Publication statusPublished - May 12 2013

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Chromosomes, Human, 16-18
Dynamins
Clamping devices
Chromosomes
Escherichia coli
DNA
Proteins
Cells
Membranes
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A replicase clamp-binding dynamin-like protein promotes colocalization of nascent DNA strands and equipartitioning of chromosomes in E.coli. / Ozaki, Shogo; Matsuda, Yusaku; Keyamura, Kenji; Kawakami, Hironori; Noguchi, Yasunori; Kasho, Kazutoshi; Nagata, Komomo; Masuda, Tamami; Sakiyama, Yukari; Katayama, Tsutomu.

In: Cell Reports, Vol. 4, No. 5, 12.05.2013, p. 985-995.

Research output: Contribution to journalArticle

Ozaki, Shogo ; Matsuda, Yusaku ; Keyamura, Kenji ; Kawakami, Hironori ; Noguchi, Yasunori ; Kasho, Kazutoshi ; Nagata, Komomo ; Masuda, Tamami ; Sakiyama, Yukari ; Katayama, Tsutomu. / A replicase clamp-binding dynamin-like protein promotes colocalization of nascent DNA strands and equipartitioning of chromosomes in E.coli. In: Cell Reports. 2013 ; Vol. 4, No. 5. pp. 985-995.
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