A Ribbon-like Structure in the Ejective Organelle of the Green Microalga Pyramimonas parkeae (Prasinophyceae) Consists of Core Histones and Polymers Containing N-acetyl-glucosamine

Takahiro Yamagishi, Akira Kurihara, Hiroshi Kawai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The green microalga, Pyramimonas parkeae (Prasinophyceae) has an ejective organelle containing a coiled ribbon structure resembling the ejectisome in Cryptophyta. This structure is discharged from the cell by a stimulus and extends to form a tube-like structure, but the molecular components of the structure have not been identified. Tricine-SDS-PAGE analysis indicated that the ribbon-like structure of P. parkeae contains some proteins and low molecular acidic polymers. Edman degradation, LC/MS/MS analyses and immunological studies demonstrated that their proteins are core histones (H3, H2A, H2B and H4). In addition, monosaccharide composition analysis of the ribbon-like structures and degradation by lysozyme strongly indicated that the ribbon-like structure consist of β (1-4) linked polymers containing N-acetyl-glucosamine. Purified polymers and recombinant histones formed glob-like or filamentous structures. Therefore we conclude that the ribbon-like structure of P. parkeae mainly consists of a complex of core histones (H3, H2A, H2B and H4) and polymers containing N-acetyl-glucosamine, and suggest to name the ejective organelle in P. parkeae the "histrosome" to distinguish it from the ejectisome in Cryptophyta.

Original languageEnglish
Pages (from-to)522-533
Number of pages12
JournalProtist
Volume166
Issue number5
DOIs
Publication statusPublished - Nov 1 2015

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Glucosamine
Organelles
Histones
Polymers
Cryptophyta
Monosaccharides
Muramidase
Molecular Structure
Names
Polyacrylamide Gel Electrophoresis
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

A Ribbon-like Structure in the Ejective Organelle of the Green Microalga Pyramimonas parkeae (Prasinophyceae) Consists of Core Histones and Polymers Containing N-acetyl-glucosamine. / Yamagishi, Takahiro; Kurihara, Akira; Kawai, Hiroshi.

In: Protist, Vol. 166, No. 5, 01.11.2015, p. 522-533.

Research output: Contribution to journalArticle

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