A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation

Ryuichi Tatsumi, Adam L. Wuollet, Kuniko Tabata, Shotaro Nishimura, Shoji Tabata, Wataru Mizunoya, Yoshihide Ikeuchi, Ronald E. Allen

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

When skeletal muscle is stretched or injured, myogenic satellite cells are activated to enter the cell cycle. This process depends on nitric oxide (NO) production by NO synthase (NOS), matrix metalloproteinase activation, release of hepatocyte growth factor (HGF) from the extracellular matrix, and presentation of HGF to the c-met receptor as demonstrated by a primary culture and in vivo assays. We now add evidence that calcium-calmodulin is involved in the satellite cell activation cascade in vitro. Conditioned medium from cultures that were treated with a calcium ionophore (A23187, ionomycin) for 2 h activated cultured satellite cells and contained active HGF, similar to the effect of mechanical stretch or NO donor treatments. The response was abolished by addition of calmodulin inhibitors (calmidazolium, W-13, W-12) or a NOS inhibitor N G-nitro-L-arginine methyl ester hydrochloride but not by its less inactive enantiomer NG-nitro-D-arginine methyl ester hydrochloride. Satellite cells were also shown to express functional calmodulin protein having a calcium-binding activity at 12 h postplating, which is the time at which the calcium ionophore was added in this study and the stretch treatment was applied in our previous experiments. Therefore, results from these experiments provide an additional insight that calcium-calmodulin mediates HGF release from the matrix and that this step in the activation pathway is upstream from NO synthesis.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume296
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

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Skeletal Muscle Satellite Cells
Hepatocyte Growth Factor
Calmodulin
Nitric Oxide
Calcium
Calcium Ionophores
Nitric Oxide Synthase
calmidazolium
Ionomycin
Nitric Oxide Donors
NG-Nitroarginine Methyl Ester
Calcimycin
Conditioned Culture Medium
Matrix Metalloproteinases
Extracellular Matrix
Cultured Cells
Cell Cycle
Skeletal Muscle
Proteins

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Physiology

Cite this

A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation. / Tatsumi, Ryuichi; Wuollet, Adam L.; Tabata, Kuniko; Nishimura, Shotaro; Tabata, Shoji; Mizunoya, Wataru; Ikeuchi, Yoshihide; Allen, Ronald E.

In: American Journal of Physiology - Cell Physiology, Vol. 296, No. 4, 01.04.2009.

Research output: Contribution to journalArticle

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