A role for the cysteine-rich 10 kDa prolamin in protein body i formation in rice

Ai Nagamine, Hiroaki Matsusaka, Tomokazu Ushijima, Yasushi Kawagoe, Masahiro Ogawa, Thomas W. Okita, Toshihiro Kumamaru

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The rice prolamins consist of cysteine-rich 10 kDa (CysR10), 14 kDa (CysR14) and 16 kDa (CysR16) molecular species and a cysteine-poor 13 kDa (CysP13) polypeptide. These storage proteins form protein bodies (PBs) composed of single spherical intracisternal inclusions assembled within the lumen of the rough endoplasmic reticulum. Immunofluorescence and immunoelectron microscopy demonstrated that CysR10 and CysP13 were asymmetrically distributed within the PBs, with the former concentrated at the electron-dense center core region and the latter distributed mainly to the electron-lucent peripheral region. These results together with temporal expression data showed that the formation of prolamin-containing PB-I in the wild-type endosperm was initiated by the accumulation of CysR10 to form the center core. In mutants deficient for cysteine-rich prolamins, the typical PB-I structures containing the electron-dense center core were not observed, and instead were replaced by irregularly shaped, electron-lucent, hypertrophied PBs. Similar, deformed PBs were observed in a CysR10 RNA interference plant line. These results suggest that CysR10, through its formation of the central core and its possible interaction with other cysteine-rich prolamins, is required for tight packaging of the proteins into a compact spherical structure.

Original languageEnglish
Pages (from-to)1003-1016
Number of pages14
JournalPlant and Cell Physiology
Volume52
Issue number6
DOIs
Publication statusPublished - Jun 2011

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'A role for the cysteine-rich 10 kDa prolamin in protein body i formation in rice'. Together they form a unique fingerprint.

Cite this