A Sapecin Homologue of Holotrichia Diomphalia: Purification, Sequencing and Determination of Disulfide Pairs

So Lee, Hyun Moon, Bok Lee, Shun-Ichiro Kawabata, Shunji Natori, Shoichiro Kurata

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We purified and characterized a sapecin homologue, named holotricin 1, from the hemolymph of immunized larvae of a coleopteran insect, Holotrichia diomphalia. We determined its complete amino acid sequence and three disulfide pairs. Holotricin 1 consisted of 43 amino acid residues and showed potent antibacterial activity against gram-positive bacteria, but antibacterial activity against gram-negative bacteria was not obvious.

Original languageEnglish
Pages (from-to)457-459
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

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Hemolymph
Gram-Positive Bacteria
Gram-Negative Bacteria
Disulfides
Larva
Insects
Amino Acid Sequence
Amino Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

A Sapecin Homologue of Holotrichia Diomphalia : Purification, Sequencing and Determination of Disulfide Pairs. / Lee, So; Moon, Hyun; Lee, Bok; Kawabata, Shun-Ichiro; Natori, Shunji; Kurata, Shoichiro.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 3, 01.01.1995, p. 457-459.

Research output: Contribution to journalArticle

Lee, So ; Moon, Hyun ; Lee, Bok ; Kawabata, Shun-Ichiro ; Natori, Shunji ; Kurata, Shoichiro. / A Sapecin Homologue of Holotrichia Diomphalia : Purification, Sequencing and Determination of Disulfide Pairs. In: Biological and Pharmaceutical Bulletin. 1995 ; Vol. 18, No. 3. pp. 457-459.
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