A selective increase in phosphorylation of cyclic AMP response element-binding protein in hippocampal CA1 region of male, but not female, rats following contextual fear and passive avoidance conditioning

Koutarou Kudo, Chun Xiang Qiao, Shigenobu Kanba, Jun Arita

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Cyclic AMP response element-binding protein (CREB), a transcription factor on which multiple signal transduction pathways converge, has been implicated in long-term memory. We examined whether the sex difference in the performance of contextual fear or passive avoidance conditioning is associated with a change in the activation of CREB in the hippocampus, a neural structure important for long-term memory. The activation of CREB in different subregions within the hippocampus in male and female rats was determined immunohistochemically with an antibody that specifically recognizes the phosphorylated form of CREB (pCREB). With respect to the freezing time in contextual fear conditioning and the step-through latency in passive avoidance conditioning, male rats exhibited better performance than female rats. Phosphorylation of CREB (% pCREB) as revealed by the ratio of the pCREB-immunoreactive (pCREB-ir) cell number to the CREB-immunoreactive cell number was increased in the CA1 region, but not in CA3, CA4, or in the dentate gyrus following training for both types of conditioning in males. In females, such an increase in % pCREB was not found in any hippocampal subregion at any time after conditioning or by increasing the intensity of foot shock. Orchidectomy in males did not alter either the performance of contextual conditioning or conditioning-induced CREB phosphorylation in CA1. The close relationship between behavioral performance and CREB phosphorylation in the CA1 region suggests that hippocampal CREB is involved in the sex difference in some forms of learning and memory.

Original languageEnglish
Pages (from-to)233-243
Number of pages11
JournalBrain Research
Volume1024
Issue number1-2
DOIs
Publication statusPublished - Oct 22 2004

Fingerprint

Hippocampal CA1 Region
Cyclic AMP Response Element-Binding Protein
Fear
Phosphorylation
Long-Term Memory
Sex Characteristics
Hippocampus
Cell Count
Conditioning (Psychology)
Orchiectomy
Dentate Gyrus
Freezing
Foot
Shock
Signal Transduction
Transcription Factors
Learning

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

A selective increase in phosphorylation of cyclic AMP response element-binding protein in hippocampal CA1 region of male, but not female, rats following contextual fear and passive avoidance conditioning. / Kudo, Koutarou; Qiao, Chun Xiang; Kanba, Shigenobu; Arita, Jun.

In: Brain Research, Vol. 1024, No. 1-2, 22.10.2004, p. 233-243.

Research output: Contribution to journalArticle

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