A semiautomatic segmentation algorithm for extracting the complete structure of acini from synchrotron micro-CT images

Luosha Xiao, Toshihiro Sera, Kenichiro Koshiyama, Shigeo Wada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pulmonary acinus is the largest airway unit provided with alveoli where blood/gas exchange takes place. Understanding the complete structure of acinus is necessary to measure the pathway of gas exchange and to simulate various mechanical phenomena in the lungs. The usual manual segmentation of a complete acinus structure from their experimentally obtained images is difficult and extremely time-consuming, which hampers the statistical analysis. In this study, we develop a semiautomatic segmentation algorithm for extracting the complete structure of acinus from synchrotron micro-CT images of the closed chest of mouse lungs. The algorithm uses a combination of conventional binary image processing techniques based on the multiscale and hierarchical nature of lung structures. Specifically, larger structures are removed, while smaller structures are isolated from the image by repeatedly applying erosion and dilation operators in order, adjusting the parameter referencing to previously obtained morphometric data. A cluster of isolated acini belonging to the same terminal bronchiole is obtained without floating voxels. The extracted acinar models above 98% agree well with those extracted manually. The run time is drastically shortened compared with manual methods. These findings suggest that our method may be useful for taking samples used in the statistical analysis of acinus.

Original languageEnglish
Article number575086
JournalComputational and Mathematical Methods in Medicine
Volume2013
DOIs
Publication statusPublished - Apr 5 2013
Externally publishedYes

Fingerprint

Micro-CT
Synchrotrons
CT Image
Statistical methods
Segmentation
Gases
Lung
Binary images
Gas Exchange
Erosion
Image processing
Blood
Mechanical Phenomena
Bronchioles
Statistical Analysis
Dilatation
Thorax
Binary Image
Voxel
Dilation

All Science Journal Classification (ASJC) codes

  • Applied Mathematics
  • Modelling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)
  • Immunology and Microbiology(all)

Cite this

A semiautomatic segmentation algorithm for extracting the complete structure of acini from synchrotron micro-CT images. / Xiao, Luosha; Sera, Toshihiro; Koshiyama, Kenichiro; Wada, Shigeo.

In: Computational and Mathematical Methods in Medicine, Vol. 2013, 575086, 05.04.2013.

Research output: Contribution to journalArticle

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