A short peptide is a protein kinase C (PKC) α-specific substrate

Jeong Hun Kang, Daisuke Asai, Satoshi Yamada, Riki Toita, Jun Oishi, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The purpose of this study was to find protein kinase C (PKC) isozyme-specific peptides. A peptide library containing 1772 sequences was designed using Scansite and screened by MALDI-TOF MS and kinase activity assays for PKC isozyme-specificity. A peptide (Alphatomega; H-FKKQGSFAKKK-NH 2) with high specificity for PKCα relative to other isozymes was identified. The peptide was phosphorylated to a greater extent by tissue lysates from B16 melanoma, HepG2, and human breast cancer, which had higher levels of activated PKCα, when compared to normal skin, liver, and human breast tissue lysates, respectively. Moreover, addition of Ro-31-7549, an inhibitor with great specificity for PKCα, to the phosphorylation reaction caused a dose-dependent reduction in phosphorylation, but no inhibition was identified with the addition of rottlerin and H-89. These results show that this peptide has great potential as a PKCα-specific substrate.

Original languageEnglish
Pages (from-to)2006-2011
Number of pages6
JournalProteomics
Volume8
Issue number10
DOIs
Publication statusPublished - May 1 2008

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Protein Kinase C
Peptides
Substrates
Isoenzymes
Phosphorylation
Tissue
Peptide Library
Experimental Melanomas
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Liver
Assays
Skin
Breast
Phosphotransferases
Breast Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

A short peptide is a protein kinase C (PKC) α-specific substrate. / Kang, Jeong Hun; Asai, Daisuke; Yamada, Satoshi; Toita, Riki; Oishi, Jun; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

In: Proteomics, Vol. 8, No. 10, 01.05.2008, p. 2006-2011.

Research output: Contribution to journalArticle

Kang, JH, Asai, D, Yamada, S, Toita, R, Oishi, J, Mori, T, Niidome, T & Katayama, Y 2008, 'A short peptide is a protein kinase C (PKC) α-specific substrate', Proteomics, vol. 8, no. 10, pp. 2006-2011. https://doi.org/10.1002/pmic.200701045
Kang JH, Asai D, Yamada S, Toita R, Oishi J, Mori T et al. A short peptide is a protein kinase C (PKC) α-specific substrate. Proteomics. 2008 May 1;8(10):2006-2011. https://doi.org/10.1002/pmic.200701045
Kang, Jeong Hun ; Asai, Daisuke ; Yamada, Satoshi ; Toita, Riki ; Oishi, Jun ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki. / A short peptide is a protein kinase C (PKC) α-specific substrate. In: Proteomics. 2008 ; Vol. 8, No. 10. pp. 2006-2011.
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AU - Mori, Takeshi

AU - Niidome, Takuro

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