A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

Hiroyuki Tanaka; Waraporn Putalun; Chiyumi Tsuzaki; Yukihiro Shoyama

doi:10.1016/S0014-5793(97)00106-3

A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

Hiroyuki Tanaka, Waraporn Putalun, Chiyumi Tsuzaki, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

Original language	English
Pages (from-to)	279-282
Number of pages	4
Journal	FEBS Letters
Volume	404
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(97)00106-3
Publication status	Published - Mar 10 1997

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(97)00106-3

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title = "A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine",

abstract = "A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.",

author = "Hiroyuki Tanaka and Waraporn Putalun and Chiyumi Tsuzaki and Yukihiro Shoyama",

note = "Funding Information: We thank Prof. T. Nohara and S. Yahara, Faculty of Pharmaceutical Sciences, Kumamoto University, for providing samples of steroidal alkaloids. Our thanks are also due to Dr. S. Morimoto, Faculty of Pharmaceutical Sciences, Kyushu University for helpful discussions. The research in this paper was supported in part by a Grant-in-Aid (No. 08457586 for Y.S.) from the Ministry of Education, Science and Culture of Japan.",

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doi = "10.1016/S0014-5793(97)00106-3",

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T1 - A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

AU - Tsuzaki, Chiyumi

AU - Shoyama, Yukihiro

N1 - Funding Information: We thank Prof. T. Nohara and S. Yahara, Faculty of Pharmaceutical Sciences, Kumamoto University, for providing samples of steroidal alkaloids. Our thanks are also due to Dr. S. Morimoto, Faculty of Pharmaceutical Sciences, Kyushu University for helpful discussions. The research in this paper was supported in part by a Grant-in-Aid (No. 08457586 for Y.S.) from the Ministry of Education, Science and Culture of Japan.

PY - 1997/3/10

Y1 - 1997/3/10

N2 - A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

AB - A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

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A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

Abstract

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