A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

Hiroyuki Tanaka, Waraporn Putalun, Chiyumi Tsuzaki, Yukihiro Shoyama

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
Publication statusPublished - Mar 10 1997

Fingerprint

Thin layer chromatography
Glycosides
Thin Layer Chromatography
Alkaloids
Monoclonal Antibodies
Bovine Serum Albumin
Membranes
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Silica Gel
Ionization
Gel Chromatography
Mass spectrometry
Desorption
solasodine
beta-solamarine
Staining and Labeling
Lasers

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine. / Tanaka, Hiroyuki; Putalun, Waraporn; Tsuzaki, Chiyumi; Shoyama, Yukihiro.

In: FEBS Letters, Vol. 404, No. 2-3, 10.03.1997, p. 279-282.

Research output: Contribution to journalArticle

Tanaka, Hiroyuki ; Putalun, Waraporn ; Tsuzaki, Chiyumi ; Shoyama, Yukihiro. / A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine. In: FEBS Letters. 1997 ; Vol. 404, No. 2-3. pp. 279-282.
@article{a095539e3199408a985e3d6ed8f2c1bb,
title = "A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine",
abstract = "A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.",
author = "Hiroyuki Tanaka and Waraporn Putalun and Chiyumi Tsuzaki and Yukihiro Shoyama",
year = "1997",
month = "3",
day = "10",
doi = "10.1016/S0014-5793(97)00106-3",
language = "English",
volume = "404",
pages = "279--282",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

AU - Tsuzaki, Chiyumi

AU - Shoyama, Yukihiro

PY - 1997/3/10

Y1 - 1997/3/10

N2 - A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

AB - A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

UR - http://www.scopus.com/inward/record.url?scp=0031041953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031041953&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(97)00106-3

DO - 10.1016/S0014-5793(97)00106-3

M3 - Article

C2 - 9119079

AN - SCOPUS:0031041953

VL - 404

SP - 279

EP - 282

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -