A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates

Yoji Asami, Jun Oishi, Hirotaro Kitazaki, Jumpei Kamimoto, Jeong Hun Kang, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalAnalytical Biochemistry
Volume418
Issue number1
DOIs
Publication statusPublished - Nov 1 2011

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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