A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates

Yoji Asami; Jun Oishi; Hirotaro Kitazaki; Jumpei Kamimoto; Jeong Hun Kang; Takuro Niidome; Takeshi Mori; Yoshiki Katayama

doi:10.1016/j.ab.2011.06.041

A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates

Yoji Asami, Jun Oishi, Hirotaro Kitazaki, Jumpei Kamimoto, Jeong Hun Kang, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.

Original language	English
Pages (from-to)	44-49
Number of pages	6
Journal	Analytical Biochemistry
Volume	418
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2011.06.041
Publication status	Published - Nov 1 2011

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2011.06.041

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abstract = "Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.",

author = "Yoji Asami and Jun Oishi and Hirotaro Kitazaki and Jumpei Kamimoto and Kang, {Jeong Hun} and Takuro Niidome and Takeshi Mori and Yoshiki Katayama",

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AU - Asami, Yoji

AU - Oishi, Jun

AU - Kitazaki, Hirotaro

AU - Kamimoto, Jumpei

AU - Kang, Jeong Hun

AU - Niidome, Takuro

AU - Mori, Takeshi

AU - Katayama, Yoshiki

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan .

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A simple set-and-mix assay for screening of protein kinase inhibitors in cell lysates

Abstract

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