A simplified method for cryopreservation of peripheral blood stem cells at -80°C without rate-controlled freezing

S. Makino, M. Harada, Koichi Akashi, S. Taniguchi, T. Shibuya, S. Inaba, Y. Niho

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117 Citations (Scopus)

Abstract

A simplified method was established for cryopreservation of peripheral blood stem cells (PBSCs) using hydroxyethyl starch (HES) and dimethylsulfoxide (DMSO) as a cryoprotective agent at -80°C without rate-controlled freezing. The data indicate that a cryoprotective solution consisting of 6% HES and 5% DMSO produced the highest recovery rates for nucleated cells (92.0 ± 3.5%), CFU-GM (73.8 ± 4.1%) and BFU-E (82.2 ± 6.9%), and the highest trypan blue viability (88.4 ± 3.6%). For long-term cryopreservation of PBSCs, CFU-GM recovery rates remained almost unchanged during 5-18 months; the mean CFU-GM recovery rate after 18 months of cryopreservation was 70 ± 11%. When large-scale samples of PBSCs in 100-ml freezing bags were cryopreserved for clinical use, cell and CFU-GM recoveries were similar. Using this method, 10 patients with hematological malignancy received PBSC transplants after marrow-ablative chemotherapy. All demonstrated early engraftment; seven are now alive in unmaintained complete remission for 3.5 - 15 months after PBSC transplant. This simple and inexpensive method will be useful for the wider application of PBSC transplant as a therapeutic alternative in the treatment of malignant diseases.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalBone Marrow Transplantation
Volume8
Issue number4
Publication statusPublished - Jan 1 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

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