A simplified method for cryopreservation of peripheral blood stem cells at -80°C without rate-controlled freezing

S. Makino, M. Harada, Koichi Akashi, S. Taniguchi, T. Shibuya, S. Inaba, Y. Niho

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

A simplified method was established for cryopreservation of peripheral blood stem cells (PBSCs) using hydroxyethyl starch (HES) and dimethylsulfoxide (DMSO) as a cryoprotective agent at -80°C without rate-controlled freezing. The data indicate that a cryoprotective solution consisting of 6% HES and 5% DMSO produced the highest recovery rates for nucleated cells (92.0 ± 3.5%), CFU-GM (73.8 ± 4.1%) and BFU-E (82.2 ± 6.9%), and the highest trypan blue viability (88.4 ± 3.6%). For long-term cryopreservation of PBSCs, CFU-GM recovery rates remained almost unchanged during 5-18 months; the mean CFU-GM recovery rate after 18 months of cryopreservation was 70 ± 11%. When large-scale samples of PBSCs in 100-ml freezing bags were cryopreserved for clinical use, cell and CFU-GM recoveries were similar. Using this method, 10 patients with hematological malignancy received PBSC transplants after marrow-ablative chemotherapy. All demonstrated early engraftment; seven are now alive in unmaintained complete remission for 3.5 - 15 months after PBSC transplant. This simple and inexpensive method will be useful for the wider application of PBSC transplant as a therapeutic alternative in the treatment of malignant diseases.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalBone Marrow Transplantation
Volume8
Issue number4
Publication statusPublished - Jan 1 1991
Externally publishedYes

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Cryopreservation
Freezing
Granulocyte-Macrophage Progenitor Cells
Dimethyl Sulfoxide
Transplants
Starch
Cryoprotective Agents
Erythroid Precursor Cells
Trypan Blue
Hematologic Neoplasms
Peripheral Blood Stem Cells
Bone Marrow
Drug Therapy
Therapeutics

All Science Journal Classification (ASJC) codes

  • Hematology
  • Transplantation

Cite this

A simplified method for cryopreservation of peripheral blood stem cells at -80°C without rate-controlled freezing. / Makino, S.; Harada, M.; Akashi, Koichi; Taniguchi, S.; Shibuya, T.; Inaba, S.; Niho, Y.

In: Bone Marrow Transplantation, Vol. 8, No. 4, 01.01.1991, p. 239-244.

Research output: Contribution to journalArticle

Makino, S, Harada, M, Akashi, K, Taniguchi, S, Shibuya, T, Inaba, S & Niho, Y 1991, 'A simplified method for cryopreservation of peripheral blood stem cells at -80°C without rate-controlled freezing', Bone Marrow Transplantation, vol. 8, no. 4, pp. 239-244.
Makino, S. ; Harada, M. ; Akashi, Koichi ; Taniguchi, S. ; Shibuya, T. ; Inaba, S. ; Niho, Y. / A simplified method for cryopreservation of peripheral blood stem cells at -80°C without rate-controlled freezing. In: Bone Marrow Transplantation. 1991 ; Vol. 8, No. 4. pp. 239-244.
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