We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - Jan 29 1986|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology