Abstract
We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.
| Original language | English |
|---|---|
| Pages (from-to) | 512-518 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 134 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 29 1986 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
A simultaneous quantitation of leukotriene B4 and its ω-oxidized products by gas chromatography-mass spectrometry. / Izumi, Takashi; Shimizu, Takao; Kasama, Takeshi; Seyama, Yousuke; Sumimoto, Hideki; Takeshige, Koichiro; Minakami, Shigeki; Wetterholm, Anders; Rådmark, Olof.
In: Biochemical and Biophysical Research Communications, Vol. 134, No. 2, 29.01.1986, p. 512-518.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A simultaneous quantitation of leukotriene B4 and its ω-oxidized products by gas chromatography-mass spectrometry
AU - Izumi, Takashi
AU - Shimizu, Takao
AU - Kasama, Takeshi
AU - Seyama, Yousuke
AU - Sumimoto, Hideki
AU - Takeshige, Koichiro
AU - Minakami, Shigeki
AU - Wetterholm, Anders
AU - Rådmark, Olof
PY - 1986/1/29
Y1 - 1986/1/29
N2 - We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.
AB - We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.
UR - http://www.scopus.com/inward/record.url?scp=0022444994&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022444994&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(86)80450-8
DO - 10.1016/S0006-291X(86)80450-8
M3 - Article
VL - 134
SP - 512
EP - 518
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -