A simultaneous quantitation of leukotriene B4 and its ω-oxidized products by gas chromatography-mass spectrometry

Takashi Izumi; Takao Shimizu; Takeshi Kasama; Yousuke Seyama; Hideki Sumimoto; Koichiro Takeshige; Shigeki Minakami; Anders Wetterholm; Olof Rådmark

doi:10.1016/S0006-291X(86)80450-8

A simultaneous quantitation of leukotriene B₄ and its ω-oxidized products by gas chromatography-mass spectrometry

Takashi Izumi, Takao Shimizu, Takeshi Kasama, Yousuke Seyama, Hideki Sumimoto, Koichiro Takeshige, Shigeki Minakami, Anders Wetterholm, Olof Rådmark

Department of Basic Medicine

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B₄ (LTB₄) and its ω-oxidized metabolites (20-hydroxy-LTB₄ and 20-carboxy-LTB₄) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB₄ and its metabolites were measured by selective ion monitorings. The detection limit of LTB₄ was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB₄ in human polymorphonuclear leukocytes were investigated.

Original language	English
Pages (from-to)	512-518
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	134
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(86)80450-8
Publication status	Published - Jan 29 1986

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(86)80450-8

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title = "A simultaneous quantitation of leukotriene B4 and its ω-oxidized products by gas chromatography-mass spectrometry",

abstract = "We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.",

author = "Takashi Izumi and Takao Shimizu and Takeshi Kasama and Yousuke Seyama and Hideki Sumimoto and Koichiro Takeshige and Shigeki Minakami and Anders Wetterholm and Olof R{\aa}dmark",

note = "Funding Information: suggestions. This work was supported in part by a grant-in-aid for Funding Information: Japan, by grants from Foundation for Cancer and Cardiovascular Diseases, Funding Information: Scientific Research from the Ministry of Education, Science and Culture of",

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doi = "10.1016/S0006-291X(86)80450-8",

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AU - Izumi, Takashi

AU - Shimizu, Takao

AU - Kasama, Takeshi

AU - Seyama, Yousuke

AU - Sumimoto, Hideki

AU - Takeshige, Koichiro

AU - Minakami, Shigeki

AU - Wetterholm, Anders

AU - Rådmark, Olof

N1 - Funding Information: suggestions. This work was supported in part by a grant-in-aid for Funding Information: Japan, by grants from Foundation for Cancer and Cardiovascular Diseases, Funding Information: Scientific Research from the Ministry of Education, Science and Culture of

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Y1 - 1986/1/29

N2 - We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.

AB - We developed a highly sensitive and specific method for the simultaneous quantitation of leukotriene B4 (LTB4) and its ω-oxidized metabolites (20-hydroxy-LTB4 and 20-carboxy-LTB4) by mass fragmentography using deuterated compounds as internal standards. The ions produced by the cleavage of the C12-13 bond of the methyl ester dimethylisopropylsilyl ether derivatives of LTB4 and its metabolites were measured by selective ion monitorings. The detection limit of LTB4 was less than 10 pg and about 100-fold lower than that by high performance liquid chromatography. By using this method, the synthesis and further metabolism of LTB4 in human polymorphonuclear leukocytes were investigated.

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A simultaneous quantitation of leukotriene B₄ and its ω-oxidized products by gas chromatography-mass spectrometry

Abstract

All Science Journal Classification (ASJC) codes

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