TY - JOUR
T1 - A single amino acid at N-terminal region of the 2b protein of cucumber mosaic virus strain m1 has a pivotal role in virus attenuation
AU - Maneechoat, Phoowanarth
AU - Takeshita, Minoru
AU - Uenoyama, Misa
AU - Nakatsukasa, Maki
AU - Kuroda, Atsuko
AU - Furuya, Naruto
AU - Tsuchiya, Kenichi
N1 - Funding Information:
We thank Prof. Masuta C., Hokkaido University, for providing C2-H1 plasmid and his suggestions. We also thank Prof. Palukaitis P., Seoul Women's University, for his critical reading. This study was partially funded by a Grant-in-Aid for scientific research (C) ( 24580067 ) from Japan Society for the Promotion of Science .
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/2/2
Y1 - 2015/2/2
N2 - Host responses to infection by a mild strain of cucumber mosaic virus, termed CMV-m1, were re-examined in several plant species in comparison with those by a severe strain CMV-Y. Mild systemic symptoms were developed on the six plant species inoculated with CMV-m1. Virus titer in the Nicotiana benthamiana plants infected with CMV-m1 was significantly lower than those infected with CMV-Y, although infection by CMV-m1 interfered with further infection by CMV-Y in the plants. Subsequently, the attenuated virulence of CMV-m1 was analyzed by reassortment and recombination analyses between CMV-m1 and CMV-Y RNAs. The results suggested that the 2b protein of CMV-m1 (m1-2b) is involved in the formation of mild symptoms in N. benthamiana. Furthermore, site-directed mutagenesis demonstrated that Thr18 of m1-2b is responsible for formation of mild symptoms. Local RNA silencing suppressor activity of m1-2b was a little lower than that of severe strain CMV-Y. We discuss the relationship between attenuation of CMV-m1 and the features of m1-2b.
AB - Host responses to infection by a mild strain of cucumber mosaic virus, termed CMV-m1, were re-examined in several plant species in comparison with those by a severe strain CMV-Y. Mild systemic symptoms were developed on the six plant species inoculated with CMV-m1. Virus titer in the Nicotiana benthamiana plants infected with CMV-m1 was significantly lower than those infected with CMV-Y, although infection by CMV-m1 interfered with further infection by CMV-Y in the plants. Subsequently, the attenuated virulence of CMV-m1 was analyzed by reassortment and recombination analyses between CMV-m1 and CMV-Y RNAs. The results suggested that the 2b protein of CMV-m1 (m1-2b) is involved in the formation of mild symptoms in N. benthamiana. Furthermore, site-directed mutagenesis demonstrated that Thr18 of m1-2b is responsible for formation of mild symptoms. Local RNA silencing suppressor activity of m1-2b was a little lower than that of severe strain CMV-Y. We discuss the relationship between attenuation of CMV-m1 and the features of m1-2b.
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U2 - 10.1016/j.virusres.2014.12.020
DO - 10.1016/j.virusres.2014.12.020
M3 - Article
C2 - 25541532
AN - SCOPUS:84922809621
SN - 0168-1702
VL - 197
SP - 67
EP - 74
JO - Virus Research
JF - Virus Research
ER -
