A single cell extraction chip using vibration-induced whirling flow and a thermo-responsive gel pattern

Takeshi Hayakawa, Shinya Sakuma, Takeshi Fukuhara, Yoshiyuki Yokoyama, Fumihito Arai

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

We propose a single cell extraction chip with an open structure, which utilizes vibration-induced whirling flow and a single cell catcher. By applying a circular vibration to a micropillar array spiral pattern, a whirling flow is induced around the micropillars, and target cells are transported towards the single cell catcher placed at the center of the spiral. The single cell catcher is composed of a single-cell-sized hole pattern of thermo-responsive gel. The gel swells at low temperatures (≲32 °C) and shrinks at high temperatures (≳32 °C), therefore, its volume expansion can be controlled by an integrated microheater. When the microheater is turned on, a single cell is trapped by the hole pattern of the single cell catcher. Then, when the microheater is turned off, the single cell catcher is cooled by the ambient temperature. The gel swells at this temperature, and the hole closes to catch the single cell. The caught cell can then be released into culture wells on a microtiter plate by heating the gel again. We conducted single cell extraction with the proposed chip and achieved a 60% success rate, of which 61% cells yielded live cells.

Original languageEnglish
Pages (from-to)681-696
Number of pages16
JournalMicromachines
Volume5
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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