A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane

Noritada Kaji, Ryo Koyama, Takao Yasui, Tetsuya Higashiyama, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a microchamber array device was designed to capture a single cell, lyse a cell membrane, and isolate a single cell nucleus, and then mRNA in a single cell nucleus were directly quantitated by using highly sensitive In-Stem Molecular Beacons (ISMB). As a result, the expression of three frequently used house-keeping genes were successfully quantitated to an accuracy of 100 molecules within a single cell nucleus.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages832-834
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Fingerprint

Cells
Membranes
Molecules
Cell membranes
Genes
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Kaji, N., Koyama, R., Yasui, T., Higashiyama, T., & Baba, Y. (2015). A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 832-834). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane. / Kaji, Noritada; Koyama, Ryo; Yasui, Takao; Higashiyama, Tetsuya; Baba, Yoshinobu.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 832-834 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kaji, N, Koyama, R, Yasui, T, Higashiyama, T & Baba, Y 2015, A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 832-834, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 10/25/15.
Kaji N, Koyama R, Yasui T, Higashiyama T, Baba Y. A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 832-834. (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Kaji, Noritada ; Koyama, Ryo ; Yasui, Takao ; Higashiyama, Tetsuya ; Baba, Yoshinobu. / A single cell nucleus array to monitor messenger-RNA molecule transportation through nuclear membrane. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 832-834 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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