A single fluorophore-labeled aptamer sensor for the detection of interferon gamma

Siti Norulhuda Hashim, Akira Tsuchiya, Noriho Kamiya, Shinsuke Sando

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Interferon gamma (IFN-γ) is a cytokine that is mainly secreted from immune cells. It plays an important role in regulating neighboring cellular activities through interactions with cell membrane proteins. Because of its biological importance, there has been increasing demand for a biosensor that can respond to IFN-γ. Here, we report a fluorescent aptamer sensor that targets IFN-γ. The 25-nt IFN-γ aptamer was converted into a turn-on fluorescent sensor by labeling the 3'-end with fluorescein. The designed sensor achieved fluorescence sensing of IFN-γ with nanomolar affinity.

Original languageEnglish
Pages (from-to)1670-1672
Number of pages3
JournalChemistry Letters
Volume44
Issue number12
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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