Abstract
The temperature-sensitive BHK21 hamster cell line tsBN67 ceases to proliferate at the nonpermissive temperature after a lag of one to a few cell divisions, and the arrested cells display a gene expression pattern similar to that of serum-starved cells. The temperature-sensitive phenotype is reversible and results from a single missense mutation-proline to serine at position 134-in HCF, a cellular protein that, together with the viral protein VP16, activates transcription of herpes simplex virus (HSV) immediate-early genes. The tsBN67 HCF mutation also prevents VP16 activation of transcription at the nonpermissive temperature. The finding that the same point mutation in HCF disrupts both VP16 function and the cell cycle suggests that HCF plays a role in cell-cycle progression in addition to VP16-dependent transcription.
| Original language | English |
|---|---|
| Pages (from-to) | 726-737 |
| Number of pages | 12 |
| Journal | Genes and Development |
| Volume | 11 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Mar 15 1997 |
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All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology
Cite this
A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function. / Goto, Hiroshige; Motomura, Seiichi; Wilson, Angus C.; Freiman, Richard N.; Nakabeppu, Yusaku; Fukushima, Kohtaro; Fujishima, Masatoshi; Herr, Winship; Nishimoto, Takeharu.
In: Genes and Development, Vol. 11, No. 6, 15.03.1997, p. 726-737.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function
AU - Goto, Hiroshige
AU - Motomura, Seiichi
AU - Wilson, Angus C.
AU - Freiman, Richard N.
AU - Nakabeppu, Yusaku
AU - Fukushima, Kohtaro
AU - Fujishima, Masatoshi
AU - Herr, Winship
AU - Nishimoto, Takeharu
PY - 1997/3/15
Y1 - 1997/3/15
N2 - The temperature-sensitive BHK21 hamster cell line tsBN67 ceases to proliferate at the nonpermissive temperature after a lag of one to a few cell divisions, and the arrested cells display a gene expression pattern similar to that of serum-starved cells. The temperature-sensitive phenotype is reversible and results from a single missense mutation-proline to serine at position 134-in HCF, a cellular protein that, together with the viral protein VP16, activates transcription of herpes simplex virus (HSV) immediate-early genes. The tsBN67 HCF mutation also prevents VP16 activation of transcription at the nonpermissive temperature. The finding that the same point mutation in HCF disrupts both VP16 function and the cell cycle suggests that HCF plays a role in cell-cycle progression in addition to VP16-dependent transcription.
AB - The temperature-sensitive BHK21 hamster cell line tsBN67 ceases to proliferate at the nonpermissive temperature after a lag of one to a few cell divisions, and the arrested cells display a gene expression pattern similar to that of serum-starved cells. The temperature-sensitive phenotype is reversible and results from a single missense mutation-proline to serine at position 134-in HCF, a cellular protein that, together with the viral protein VP16, activates transcription of herpes simplex virus (HSV) immediate-early genes. The tsBN67 HCF mutation also prevents VP16 activation of transcription at the nonpermissive temperature. The finding that the same point mutation in HCF disrupts both VP16 function and the cell cycle suggests that HCF plays a role in cell-cycle progression in addition to VP16-dependent transcription.
UR - http://www.scopus.com/inward/record.url?scp=0030915297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030915297&partnerID=8YFLogxK
U2 - 10.1101/gad.11.6.726
DO - 10.1101/gad.11.6.726
M3 - Article
C2 - 9087427
AN - SCOPUS:0030915297
VL - 11
SP - 726
EP - 737
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 6
ER -