A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function

Hiroshige Goto, Seiichi Motomura, Angus C. Wilson, Richard N. Freiman, Yusaku Nakabeppu, Kohtaro Fukushima, Masatoshi Fujishima, Winship Herr, Takeharu Nishimoto

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The temperature-sensitive BHK21 hamster cell line tsBN67 ceases to proliferate at the nonpermissive temperature after a lag of one to a few cell divisions, and the arrested cells display a gene expression pattern similar to that of serum-starved cells. The temperature-sensitive phenotype is reversible and results from a single missense mutation-proline to serine at position 134-in HCF, a cellular protein that, together with the viral protein VP16, activates transcription of herpes simplex virus (HSV) immediate-early genes. The tsBN67 HCF mutation also prevents VP16 activation of transcription at the nonpermissive temperature. The finding that the same point mutation in HCF disrupts both VP16 function and the cell cycle suggests that HCF plays a role in cell-cycle progression in addition to VP16-dependent transcription.

Original languageEnglish
Pages (from-to)726-737
Number of pages12
JournalGenes and Development
Volume11
Issue number6
DOIs
Publication statusPublished - Mar 15 1997

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Cell Cycle Checkpoints
Point Mutation
Temperature
Cell Cycle
Immediate-Early Genes
Viral Proteins
Missense Mutation
Simplexvirus
Proline
Cricetinae
Cell Division
Serine
Transcriptional Activation
Phenotype
Gene Expression
Cell Line
Mutation
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function. / Goto, Hiroshige; Motomura, Seiichi; Wilson, Angus C.; Freiman, Richard N.; Nakabeppu, Yusaku; Fukushima, Kohtaro; Fujishima, Masatoshi; Herr, Winship; Nishimoto, Takeharu.

In: Genes and Development, Vol. 11, No. 6, 15.03.1997, p. 726-737.

Research output: Contribution to journalArticle

Goto, H, Motomura, S, Wilson, AC, Freiman, RN, Nakabeppu, Y, Fukushima, K, Fujishima, M, Herr, W & Nishimoto, T 1997, 'A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function', Genes and Development, vol. 11, no. 6, pp. 726-737. https://doi.org/10.1101/gad.11.6.726
Goto, Hiroshige ; Motomura, Seiichi ; Wilson, Angus C. ; Freiman, Richard N. ; Nakabeppu, Yusaku ; Fukushima, Kohtaro ; Fujishima, Masatoshi ; Herr, Winship ; Nishimoto, Takeharu. / A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function. In: Genes and Development. 1997 ; Vol. 11, No. 6. pp. 726-737.
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