A solid-in-oil nanodispersion for transcutaneous protein delivery

Yoshiro Tahara, Shota Honda, Noriho Kamiya, Hongyu Piao, Akihiko Hirata, Eiji Hayakawa, Takeru Fujii, Masahiro Goto

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Transcutaneous delivery attracts much attention but remains a challenging strategy for hydrophilic macromolecular drug administration. In the present study, we demonstrated that a solid-in-oil (S/O) nanodispersion, an oil-based nanodispersion of hydrophilic drugs, effectively enhanced the permeation of proteins into the skin. All of the different model proteins, FITC-labeled insulin (MW ca. 6 kDa), enhanced green fluorescent protein (EGFP, MW ca. 27 kDa) and horseradish peroxidase (HRP, MW ca. 40 kDa), permeated through the stratum corneum of Yucatan micropig skin in vitro by forming a S/O nanodispersion. The penetrated EGFP and HRP exhibited green fluorescence and catalytic activity, respectively, suggesting that these proteins can permeate into the skin in a functional form. The results indicated the potential utility of the S/O nanodispersion as a novel vehicle for transcutaneous protein delivery.

Original languageEnglish
Pages (from-to)14-18
Number of pages5
JournalJournal of Controlled Release
Volume131
Issue number1
DOIs
Publication statusPublished - Oct 6 2008

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Oils
Skin
Proteins
Horseradish Peroxidase
Pharmaceutical Preparations
Cornea
Fluorescence

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

A solid-in-oil nanodispersion for transcutaneous protein delivery. / Tahara, Yoshiro; Honda, Shota; Kamiya, Noriho; Piao, Hongyu; Hirata, Akihiko; Hayakawa, Eiji; Fujii, Takeru; Goto, Masahiro.

In: Journal of Controlled Release, Vol. 131, No. 1, 06.10.2008, p. 14-18.

Research output: Contribution to journalArticle

Tahara, Yoshiro ; Honda, Shota ; Kamiya, Noriho ; Piao, Hongyu ; Hirata, Akihiko ; Hayakawa, Eiji ; Fujii, Takeru ; Goto, Masahiro. / A solid-in-oil nanodispersion for transcutaneous protein delivery. In: Journal of Controlled Release. 2008 ; Vol. 131, No. 1. pp. 14-18.
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AU - Hayakawa, Eiji

AU - Fujii, Takeru

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