A strategy for the determination of the elemental composition by fourier transform ion cyclotron resonance mass spectrometry based on isotopic peak ratios

Daisuke Miura, Yukiko Tsuji, Katsutoshi Takahashi, Hiroyuki Wariishi, Kazunori Saito

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

We propose a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by high-magnetic field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Using 3′-phosphoadenosine 5′-phosphosulfate and CTU guanamine as standard organic compounds, isotopic peaks derived from 15N-, 34S-, and 18O-substituted forms were separated from 13C-substituted species. Furthermore, these isotopic peaks were quantitatively detected and closely matched the natural abundance of each element. These data successfully led us to determine the one elemental composition in a standard independent manner. The approach should be particularly amenable to the metabolomics research field.

Original languageEnglish
Pages (from-to)5887-5891
Number of pages5
JournalAnalytical Chemistry
Volume82
Issue number13
DOIs
Publication statusPublished - Jul 1 2010

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Cyclotron resonance
Organic compounds
Mass spectrometry
Fourier transforms
Ions
Chemical analysis
Magnetic fields
Metabolomics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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A strategy for the determination of the elemental composition by fourier transform ion cyclotron resonance mass spectrometry based on isotopic peak ratios. / Miura, Daisuke; Tsuji, Yukiko; Takahashi, Katsutoshi; Wariishi, Hiroyuki; Saito, Kazunori.

In: Analytical Chemistry, Vol. 82, No. 13, 01.07.2010, p. 5887-5891.

Research output: Contribution to journalArticle

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