We propose a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by high-magnetic field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Using 3′-phosphoadenosine 5′-phosphosulfate and CTU guanamine as standard organic compounds, isotopic peaks derived from 15N-, 34S-, and 18O-substituted forms were separated from 13C-substituted species. Furthermore, these isotopic peaks were quantitatively detected and closely matched the natural abundance of each element. These data successfully led us to determine the one elemental composition in a standard independent manner. The approach should be particularly amenable to the metabolomics research field.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry