A structural model of the PriB–DnaT complex in Escherichia coli replication restart

Yoshito Abe, Yohei Ikeda, Saki Fujiyama, R. Manjunatha Kini, Tadashi Ueda

Research output: Contribution to journalArticlepeer-review

Abstract

In Escherichia coli, DNA replication is restarted following DNA repair by the PriA-dependent pathway, in which the binding and dissociation of proteins such as PriA, PriB, and DnaT on ssDNA lead to the formation of a protein–DNA complex for recruiting the DnaB–DnaC replication protein complex. However, the structure of the PriB–DnaT complex, which is an essential step in the PriA-dependent pathway, remains elusive. In this study, the importance of His26 in PriB for replication restart was reconfirmed using plasmid complementation. Furthermore, we used NMR to examine the DnaT interaction sites on PriB. We also evaluated the PriB–DnaT peptide complex model, which was prepared by in silico docking, using molecular dynamic simulation. From these data, we propose a structural model that provides insight into the PriB–DnaT interaction.

Original languageEnglish
Pages (from-to)341-350
Number of pages10
JournalFEBS Letters
Volume595
Issue number3
DOIs
Publication statusPublished - Feb 2021

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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