A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

John K. Cullison, Fred M. Hawkridge, Naotoshi Nakashima, Shimichi Yoshikawa

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83 Citations (Scopus)

Abstract

A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

Original languageEnglish
Pages (from-to)877-882
Number of pages6
JournalLangmuir
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 1 1994

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Cullison, J. K., Hawkridge, F. M., Nakashima, N., & Yoshikawa, S. (1994). A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces. Langmuir, 10(3), 877-882. https://doi.org/10.1021/la00015a044