A Study of Cytochrome c Oxidase in Lipid Bilayer Membranes on Electrode Surfaces

John K. Cullison, Fred M. Hawkridge, Naotoshi Nakashima, Shimichi Yoshikawa

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    89 Citations (Scopus)

    Abstract

    A membrane-associated enzyme, cytochrome c oxidase, has been put in lipid bilayer membranes attached to gold electrode surfaces. Gold/thiol self-assembly chemistry and deoxycholate dialysis procedures have been used to insert cytochrome c oxidase into a lipid bilayer on gold surfaces with a controlled orientation. Voltammetric and spectroelectrochemical results indicate that direct electron-transfer communication between the gold electrode surface and cytochrome c oxidase has been achieved. Moreover, immobilized cytochrome c oxidase can both reduce and oxidize solution-resident cytochrome c by appropriately controlling the applied electrode potential.

    Original languageEnglish
    Pages (from-to)877-882
    Number of pages6
    JournalLangmuir
    Volume10
    Issue number3
    DOIs
    Publication statusPublished - Mar 1 1994

    All Science Journal Classification (ASJC) codes

    • Materials Science(all)
    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Spectroscopy
    • Electrochemistry

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