A study on the surface hydrophobicity of lipases

Yoshiaki Sugimura, Kimitoshi Fukunaga, Takahiro Matsuno, Katsumi Nakao, Masahiro Goto, Fumiyuki Nakashio

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalBiochemical Engineering Journal
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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