A study on the surface hydrophobicity of lipases

Yoshiaki Sugimura, Kimitoshi Fukunaga, Takahiro Matsuno, Katsumi Nakao, Masahiro Goto, Fumiyuki Nakashio

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Surface properties, including surface net and local hydrophobicities, of bovine serum albumin, γ-globulin, and six lipases of different origins were evaluated using the aqueous two-phase partitioning method. Each showed a specific and characteristic pattern of surface properties. Correlations between the protein surface hydrophobicities and the coverages of the proteins by lipid-coating with a synthetic detergent, dioleyl glucosyl L- glutamate, were discussed. The results indicated that the surface net hydrophobicity of each protein was indicative of the affinity of the protein for the coating detergent applied in lipid-coating. (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalBiochemical Engineering Journal
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 2000

Fingerprint

Lipases
Hydrophobicity
Lipase
Hydrophobic and Hydrophilic Interactions
Surface Properties
Proteins
Detergents
Coatings
Surface properties
Lipids
Serum Globulins
Bovine Serum Albumin
Glutamic Acid
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

Sugimura, Y., Fukunaga, K., Matsuno, T., Nakao, K., Goto, M., & Nakashio, F. (2000). A study on the surface hydrophobicity of lipases. Biochemical Engineering Journal, 5(2), 123-128. https://doi.org/10.1016/S1369-703X(99)00072-8

A study on the surface hydrophobicity of lipases. / Sugimura, Yoshiaki; Fukunaga, Kimitoshi; Matsuno, Takahiro; Nakao, Katsumi; Goto, Masahiro; Nakashio, Fumiyuki.

In: Biochemical Engineering Journal, Vol. 5, No. 2, 01.06.2000, p. 123-128.

Research output: Contribution to journalArticle

Sugimura, Y, Fukunaga, K, Matsuno, T, Nakao, K, Goto, M & Nakashio, F 2000, 'A study on the surface hydrophobicity of lipases', Biochemical Engineering Journal, vol. 5, no. 2, pp. 123-128. https://doi.org/10.1016/S1369-703X(99)00072-8
Sugimura, Yoshiaki ; Fukunaga, Kimitoshi ; Matsuno, Takahiro ; Nakao, Katsumi ; Goto, Masahiro ; Nakashio, Fumiyuki. / A study on the surface hydrophobicity of lipases. In: Biochemical Engineering Journal. 2000 ; Vol. 5, No. 2. pp. 123-128.
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