Abstract
A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.
| Original language | English |
|---|---|
| Pages (from-to) | 2913-2919 |
| Number of pages | 7 |
| Journal | Journal of Separation Science |
| Volume | 34 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - Oct 1 2011 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Filtration and Separation
Cite this
A surface plasmon resonance sensor on a compact disk-type microfluidic device. / Hemmi, Akihide; Usui, Takashi; Moto, Akihiro; Tobita, Tatsuya; Soh, Nobuaki; Nakano, Koji; Zeng, Hulie; Uchiyama, Katsumi; Imato, Toshihiko; Nakajima, Hizuru.
In: Journal of Separation Science, Vol. 34, No. 20, 01.10.2011, p. 2913-2919.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A surface plasmon resonance sensor on a compact disk-type microfluidic device
AU - Hemmi, Akihide
AU - Usui, Takashi
AU - Moto, Akihiro
AU - Tobita, Tatsuya
AU - Soh, Nobuaki
AU - Nakano, Koji
AU - Zeng, Hulie
AU - Uchiyama, Katsumi
AU - Imato, Toshihiko
AU - Nakajima, Hizuru
PY - 2011/10/1
Y1 - 2011/10/1
N2 - A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.
AB - A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.
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UR - http://www.scopus.com/inward/citedby.url?scp=80054969243&partnerID=8YFLogxK
U2 - 10.1002/jssc.201100446
DO - 10.1002/jssc.201100446
M3 - Article
C2 - 21928434
AN - SCOPUS:80054969243
VL - 34
SP - 2913
EP - 2919
JO - Journal of Separation Science
JF - Journal of Separation Science
SN - 1615-9306
IS - 20
ER -