A surface plasmon resonance sensor on a compact disk-type microfluidic device

Akihide Hemmi, Takashi Usui, Akihiro Moto, Tatsuya Tobita, Nobuaki Soh, Koji Nakano, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato, Hizuru Nakajima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.

Original languageEnglish
Pages (from-to)2913-2919
Number of pages7
JournalJournal of Separation Science
Volume34
Issue number20
DOIs
Publication statusPublished - Oct 1 2011

Fingerprint

Surface plasmon resonance
Microfluidics
Immunoglobulin A
Sensors
polycarbonate
Polydimethylsiloxane
Polycarbonates
Microchannels
Washing
Optical systems
Refractive index
Pumps
Wavelength

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

A surface plasmon resonance sensor on a compact disk-type microfluidic device. / Hemmi, Akihide; Usui, Takashi; Moto, Akihiro; Tobita, Tatsuya; Soh, Nobuaki; Nakano, Koji; Zeng, Hulie; Uchiyama, Katsumi; Imato, Toshihiko; Nakajima, Hizuru.

In: Journal of Separation Science, Vol. 34, No. 20, 01.10.2011, p. 2913-2919.

Research output: Contribution to journalArticle

Hemmi, A, Usui, T, Moto, A, Tobita, T, Soh, N, Nakano, K, Zeng, H, Uchiyama, K, Imato, T & Nakajima, H 2011, 'A surface plasmon resonance sensor on a compact disk-type microfluidic device', Journal of Separation Science, vol. 34, no. 20, pp. 2913-2919. https://doi.org/10.1002/jssc.201100446
Hemmi, Akihide ; Usui, Takashi ; Moto, Akihiro ; Tobita, Tatsuya ; Soh, Nobuaki ; Nakano, Koji ; Zeng, Hulie ; Uchiyama, Katsumi ; Imato, Toshihiko ; Nakajima, Hizuru. / A surface plasmon resonance sensor on a compact disk-type microfluidic device. In: Journal of Separation Science. 2011 ; Vol. 34, No. 20. pp. 2913-2919.
@article{c87ce65d5f5c4ae58b85ca43259e1986,
title = "A surface plasmon resonance sensor on a compact disk-type microfluidic device",
abstract = "A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.",
author = "Akihide Hemmi and Takashi Usui and Akihiro Moto and Tatsuya Tobita and Nobuaki Soh and Koji Nakano and Hulie Zeng and Katsumi Uchiyama and Toshihiko Imato and Hizuru Nakajima",
year = "2011",
month = "10",
day = "1",
doi = "10.1002/jssc.201100446",
language = "English",
volume = "34",
pages = "2913--2919",
journal = "Journal of Separation Science",
issn = "1615-9306",
publisher = "Wiley-VCH Verlag",
number = "20",

}

TY - JOUR

T1 - A surface plasmon resonance sensor on a compact disk-type microfluidic device

AU - Hemmi, Akihide

AU - Usui, Takashi

AU - Moto, Akihiro

AU - Tobita, Tatsuya

AU - Soh, Nobuaki

AU - Nakano, Koji

AU - Zeng, Hulie

AU - Uchiyama, Katsumi

AU - Imato, Toshihiko

AU - Nakajima, Hizuru

PY - 2011/10/1

Y1 - 2011/10/1

N2 - A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.

AB - A surface plasmon resonance (SPR) sensor on a compact disk (CD)-type microfluidic device was developed to miniaturize the elements of a complete analytical system, pump and valves. The CD-type microfluidic device was fabricated by attaching a polydimethylsiloxane disk plate that contained microchannels and reservoirs to a flat polycarbonate disk plate that contained grating films with a thin layer of Au. The optical system of the SPR sensor and the theory for its operation are based on the principle of a grating coupled-type SPR. The sample and reagent solutions in the reservoirs on the CD-type microfluidic device were sequentially introduced into the detection chamber by centrifugal force generated by the rotation of the microfluidic device. The variation of resonance wavelength was dependent on the refractive index of the sample solution. This CD-type SPR sensor was successfully used in an immunoassay of immunoglobulin A (IgA). The anti-IgA, blocking reagent, sample and washing solution in the reservoirs were sequentially introduced into the detection chamber by changing the frequency of rotation of the microfluidic device. IgA in the sample solution was adsorbed to the anti-IgA immobilized on the Au thin layer in the detection chamber and was then detected by the SPR sensor.

UR - http://www.scopus.com/inward/record.url?scp=80054969243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054969243&partnerID=8YFLogxK

U2 - 10.1002/jssc.201100446

DO - 10.1002/jssc.201100446

M3 - Article

C2 - 21928434

AN - SCOPUS:80054969243

VL - 34

SP - 2913

EP - 2919

JO - Journal of Separation Science

JF - Journal of Separation Science

SN - 1615-9306

IS - 20

ER -