A survey of growth stimulators in natural rubber serum powder for Bifidobacterium bifidum

Shin Ichi Etoh, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

Abstract

Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cut off 1000). The active ultrafiltrate was furthermore concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained could not exhibit a growth-stimulating activity independently but acted complementarily with ammonium sulfate contaminated in the ultrafiltrate. Furthermore, ammonium sulfate could be substituted with other ammonium salts, ammonium chloride and ammonium nitrate. The adsorbed fraction was subsequently analyzed on reversed-phase high performance liquid chromatography, and the activities of the eluates were measured on B12 assay medium supplemented with ammonium sulfate. Consequently, it was proved that several ingredients in the adsorbed fraction enhanced the growth of B. bifidum.

Original languageEnglish
Pages (from-to)451-460
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume43
Issue number3-4
Publication statusPublished - Feb 1999

Fingerprint

Bifidobacterium bifidum
Ammonium Sulfate
rubber
ammonium sulfate
powders
Growth
ammonium salts
Ammonium Chloride
ammonium chloride
Ultrafiltration
reversed-phase high performance liquid chromatography
assays
Reverse-Phase Chromatography
ammonium nitrate
ultrafiltration
Ammonium Compounds
ingredients
crude protein
Salts
Molecular Weight

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

A survey of growth stimulators in natural rubber serum powder for Bifidobacterium bifidum. / Etoh, Shin Ichi; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 43, No. 3-4, 02.1999, p. 451-460.

Research output: Contribution to journalArticle

Etoh, Shin Ichi ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / A survey of growth stimulators in natural rubber serum powder for Bifidobacterium bifidum. In: Journal of the Faculty of Agriculture, Kyushu University. 1999 ; Vol. 43, No. 3-4. pp. 451-460.
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