TY - JOUR
T1 - A sustained increase of cytosolic ca2+ in γδ T cells triggered by co-stimulation via TCR/CD3 and LFA-1
AU - Kobayashi, N.
AU - Hiromatsu, K.
AU - Matsuzaki, G.
AU - Harada, M.
AU - Matsumoto, Y.
AU - Nomoto, K.
AU - Yoshikai, Y.
N1 - Funding Information:
We thank Dr Jeffrey A. Bluestone for providing the anti-CD3 E chain mAb (145-2C 1 l), Dr Yoshiyuki Hashimoto for providing the anti-LFA-1 CLc hain mAb (KBA), and Dr Shin-I&i Nishikawa for providing the stromal cell line (ST2). This work was supported by grants to YY for General Scientific Research from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare, and to NK from the Japanese Society for the Promotion of Science in Japan.
PY - 1997/12
Y1 - 1997/12
N2 - We previously reported that co-stimulation with LFA-1 triggered apoptosis in γδ T cells but not in αβ T cells after TCR engagement. We extended our earlier study on TCR/LFA-1 triggered apoptosis to two autoreactive TCR γδ and TCR αβ T cell clones, which were derived from syngeneic mixed lymphocyte culture of BALB/c mice. A γδ T cell clone, KM1, expressed the Vγ4 and Vδ5 genes and CD4-CD8-CD45RB+ phenotype; and an αβ T cell clone, BASL1.1, expressed Vβ6 and CD4+CD8-CD45RB+. Both clones produced Th-1-type cytokines in response to syngeneic BALB/c stimulator cells. KM1 underwent apoptosis upon stimulation with immobilized anti-CD3/LFA-1 mAbs, whereas BASL1.1 could proliferate successfully in response to stimulation with the immobilized mAbs. BASL1.1 was able to down-regulate the increased cytosolic Ca2+ after the simultaneous stimulation, but KM1 exhibited a sustained increase of cytosolic Ca2+ after stimulation via CD3 and LFA-1. Similar results with respect to the kinetics of cytosolic Ca2+ were obtained with normal heterogeneous γδ and αβ T cell populations after cc-stimulation via CD3 and LFA-1. Our results suggested that persistently high levels of cytosolic Ca2+ might be related to apoptosis in γδ T cell clone triggered by co-stimulation via CD3 and LFA-1.
AB - We previously reported that co-stimulation with LFA-1 triggered apoptosis in γδ T cells but not in αβ T cells after TCR engagement. We extended our earlier study on TCR/LFA-1 triggered apoptosis to two autoreactive TCR γδ and TCR αβ T cell clones, which were derived from syngeneic mixed lymphocyte culture of BALB/c mice. A γδ T cell clone, KM1, expressed the Vγ4 and Vδ5 genes and CD4-CD8-CD45RB+ phenotype; and an αβ T cell clone, BASL1.1, expressed Vβ6 and CD4+CD8-CD45RB+. Both clones produced Th-1-type cytokines in response to syngeneic BALB/c stimulator cells. KM1 underwent apoptosis upon stimulation with immobilized anti-CD3/LFA-1 mAbs, whereas BASL1.1 could proliferate successfully in response to stimulation with the immobilized mAbs. BASL1.1 was able to down-regulate the increased cytosolic Ca2+ after the simultaneous stimulation, but KM1 exhibited a sustained increase of cytosolic Ca2+ after stimulation via CD3 and LFA-1. Similar results with respect to the kinetics of cytosolic Ca2+ were obtained with normal heterogeneous γδ and αβ T cell populations after cc-stimulation via CD3 and LFA-1. Our results suggested that persistently high levels of cytosolic Ca2+ might be related to apoptosis in γδ T cell clone triggered by co-stimulation via CD3 and LFA-1.
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U2 - 10.1016/S0143-4160(97)90069-5
DO - 10.1016/S0143-4160(97)90069-5
M3 - Article
C2 - 9502191
AN - SCOPUS:0031442882
VL - 22
SP - 421
EP - 430
JO - Cell Calcium
JF - Cell Calcium
SN - 0143-4160
IS - 6
ER -