TY - JOUR
T1 - A systematic survey of repetitive sequences abundantly expressed in rat tumors
AU - Kohnoe, Shunji
AU - Maehara, Yoshihiko
AU - Endo, Hideya
N1 - Funding Information:
We thank M. Ohara for comment on the manuscript and N. Kinoshita and M. Honda for technical assistance. Dr. H. Sato (Sasaki Institute, Tokyo) and Kureha Institute kindly provided the tumor cell lines. This work was supported by Grants-in-Aid from the Ministry of Education, Science and Culture, and from the Japan Tobacco Incorporation.
PY - 1987/7/14
Y1 - 1987/7/14
N2 - A cDNA library made from poly(A)+ RNA of a rat ascites hepatoma cell line, AH60C, was screened with respect to transcribed repetitive sequences by probing with AH60C genomic DNA. Thirty clones, comprising 1.2% of the library, were found to contain repetitive elements. The sequences of 12 clones among them were abundantly expressed in poly(A)+ RNA of AH60C, yet sparse in that of rat normal liver. This differential expression was also noted in case of other tumors and normal tissues tested. These 12 clones could be divided into two classes, depending on whether their sequences were expressed in cytoplasmic poly(A)+ RNA (class I) or not (class II). Rat Alu-like type II (B2) repeats and an unknown repetitive sequence were found in the clones of class I. Adjacent to each of them, we detected two non-repeated sequences, the expressions of which were also activated. The presence of another unknown repetitive sequence was observed in the clones of class II. It is noted that more than one-third of the transcribed repetitive sequences were involved in the predominant expression in tumor cells, whereas the majority of overall transcripts have been found to be common to both transformed cells and their normal counterparts.
AB - A cDNA library made from poly(A)+ RNA of a rat ascites hepatoma cell line, AH60C, was screened with respect to transcribed repetitive sequences by probing with AH60C genomic DNA. Thirty clones, comprising 1.2% of the library, were found to contain repetitive elements. The sequences of 12 clones among them were abundantly expressed in poly(A)+ RNA of AH60C, yet sparse in that of rat normal liver. This differential expression was also noted in case of other tumors and normal tissues tested. These 12 clones could be divided into two classes, depending on whether their sequences were expressed in cytoplasmic poly(A)+ RNA (class I) or not (class II). Rat Alu-like type II (B2) repeats and an unknown repetitive sequence were found in the clones of class I. Adjacent to each of them, we detected two non-repeated sequences, the expressions of which were also activated. The presence of another unknown repetitive sequence was observed in the clones of class II. It is noted that more than one-third of the transcribed repetitive sequences were involved in the predominant expression in tumor cells, whereas the majority of overall transcripts have been found to be common to both transformed cells and their normal counterparts.
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U2 - 10.1016/0167-4781(87)90032-7
DO - 10.1016/0167-4781(87)90032-7
M3 - Article
C2 - 3593728
AN - SCOPUS:0023653164
SN - 0167-4781
VL - 909
SP - 107
EP - 114
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 2
ER -