A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki, Keisuke Wada, Megumi Furukawa, Katsumi Doi, Toshihisa Ohshima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

Original languageEnglish
Pages (from-to)2316-2318
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number11
DOIs
Publication statusPublished - 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426'. Together they form a unique fingerprint.

  • Cite this