A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426

Hirokazu Suzuki, Keisuke Wada, Megumi Furukawa, Katsumi Doi, Toshihisa Ohshima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This report describes efficient plasmid uptake by the thermophile Geobacillus kaustophilus HTA426 by means of a ternary conjugation system, which was used to construct thermophile DNA libraries for G. kaustophilus and to identify the genes for orotidine-5'-phosphate decarboxylase by in vivo functional screening. The results indicate that the conjugation system is useful in constructing G. kaustophilus libraries, which are practical in identifying thermophile genes.

Original languageEnglish
Pages (from-to)2316-2318
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number11
DOIs
Publication statusPublished - Dec 6 2013
Externally publishedYes

Fingerprint

Geobacillus
Gene Library
Orotidine-5'-Phosphate Decarboxylase
Genes
Libraries
Screening
Plasmids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426. / Suzuki, Hirokazu; Wada, Keisuke; Furukawa, Megumi; Doi, Katsumi; Ohshima, Toshihisa.

In: Bioscience, Biotechnology and Biochemistry, Vol. 77, No. 11, 06.12.2013, p. 2316-2318.

Research output: Contribution to journalArticle

Suzuki, Hirokazu ; Wada, Keisuke ; Furukawa, Megumi ; Doi, Katsumi ; Ohshima, Toshihisa. / A ternary conjugation system for the construction of DNA libraries for geobacillus kaustophilus HTA426. In: Bioscience, Biotechnology and Biochemistry. 2013 ; Vol. 77, No. 11. pp. 2316-2318.
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