A ti-plasmid-convertible λ phage vector system harboring the hygromycin B phosphotransferase gene

Takuichi Fuse, Hiroaki Kodama, Nobuaki Hayashida, Kazuo Shinozaki, Koh Iba

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Abstract

We constructed new λ TI phage vectors, λTI3, λTI4, λTI5 and λTI6, which were automatically convertible into Ti-plasmid binary vectors by using the Cre- lox site-specific recombination system of bacteriophage PI. Hygromycin B phosphotransferase (HPT) gene was inserted in the T-DNA region of these ATI vectors as a plant selectable maker. Tobacco plants, which were transformed by Agrobacterium containing the Ti-plasmids resulted from the automatic conversion of the λTI vectors, could be tightly selected by hygromycin B. This λTI system will certainly provide a powerful tool for genetic complementation test of candidate genes obtained by positional cloning.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalPlant Biotechnology
Volume15
Issue number4
DOIs
Publication statusPublished - Jan 1 1998

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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