We constructed new λ TI phage vectors, λTI3, λTI4, λTI5 and λTI6, which were automatically convertible into Ti-plasmid binary vectors by using the Cre- lox site-specific recombination system of bacteriophage PI. Hygromycin B phosphotransferase (HPT) gene was inserted in the T-DNA region of these ATI vectors as a plant selectable maker. Tobacco plants, which were transformed by Agrobacterium containing the Ti-plasmids resulted from the automatic conversion of the λTI vectors, could be tightly selected by hygromycin B. This λTI system will certainly provide a powerful tool for genetic complementation test of candidate genes obtained by positional cloning.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science