A tightly regulated expression system for E. coli using supersaturated silicic acid

Yasuhiro Fujino, Ryo Tanoue, Takushi Yokoyama, Katsumi Doi

Research output: Contribution to journalArticle

Abstract

Objective: To develop a new expression system regulated by a ferric uptake regulator in which silicic acid is used as an inducer. Results: Fur box (binding site for Fur) was substituted for the lac operator to regulate the expression of GFP with the lac promoter. Since the addition of supersaturated silicic acid invokes iron deficiency, supersaturated silicic acids were used as an inducer. GFP expression was dependent on silica concentration, and the expression level without silica was negligible. Basal expression level of this lac-Fur system was extremely low and, hence, lytic enzyme gene E from bacteriophage ϕX174 could be retained in this system. Furthermore, the expression of genes of interest was spontaneously initiated as the cell density increased and the costs of the inducer are considerably less than IPTG. Conclusion: The combination of lac promoter and Ferric uptake repressor allowed the protein expression by supersaturated silicic acid as an inducer in an easy and cost-effective way.

Original languageEnglish
Pages (from-to)1381-1387
Number of pages7
JournalBiotechnology letters
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

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Silicic Acid
Escherichia coli
Acids
Silicon Dioxide
Genes
Silica
Repressor Proteins
Isopropyl Thiogalactoside
Costs and Cost Analysis
Bacteriophages
Binding sites
Costs
Iron
Enzymes
Cell Count
Binding Sites
Proteins
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biotechnology

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A tightly regulated expression system for E. coli using supersaturated silicic acid. / Fujino, Yasuhiro; Tanoue, Ryo; Yokoyama, Takushi; Doi, Katsumi.

In: Biotechnology letters, Vol. 38, No. 8, 01.08.2016, p. 1381-1387.

Research output: Contribution to journalArticle

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