A tobacco microsomal ω-3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomoea batatas)

Y. Wakita, M. Otani, T. Hamada, M. Mori, Koh Iba, T. Shimada

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A tobacco microsomal ω-3 fatty acid desaturase gene (NtFAD3) under the control of the CaMV 35S promoter or an improved CaMV 35S promoter (E12ω) was introduced into sweet potato. Transformed sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. The transgenic plants grew normally to form storage roots and showed properties similar to those of the non-transgenic plants. The fatty acid composition in the transgenic line with a NtFAD3 gene driven by the CaMV 35S promoter was similar to that in the non-transformant. However, in the transgenic line that had a NtFAD3 gene driven by the E12ω promoter, linoleic acid (18:2) and linolenic acid (18:3) contents were 47.7 mol% and 24.8 mol%, respectively, which were significantly different from the 53.6 mol% and 11.3 mol%, respectively, in the non-transformant. The NtFAD3 gene driven by the E12ω promoter was expressed more strongly than that driven by the CaMV 35S promoter, thereby increasing the linolenic acid content in the transgenic sweet potato plants.

Original languageEnglish
Pages (from-to)244-249
Number of pages6
JournalPlant Cell Reports
Volume20
Issue number3
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

stearoyl-CoA desaturase
Ipomoea batatas
sweet potatoes
linolenic acid
tobacco
promoter regions
genetically modified organisms
genes
Agrobacterium radiobacter
linoleic acid
transgenic plants
callus
fatty acid composition

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

A tobacco microsomal ω-3 fatty acid desaturase gene increases the linolenic acid content in transgenic sweet potato (Ipomoea batatas). / Wakita, Y.; Otani, M.; Hamada, T.; Mori, M.; Iba, Koh; Shimada, T.

In: Plant Cell Reports, Vol. 20, No. 3, 01.01.2001, p. 244-249.

Research output: Contribution to journalArticle

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