A Toll-like receptor in horseshoe crabs

Kei Ichiro Inamori, Shigeru Ariki, Shun-Ichiro Kawabata

Research output: Contribution to journalReview article

36 Citations (Scopus)

Abstract

Non-self-recognition of invading microbes relies on the pattern-recognition of pathogen-associated molecular patterns (PAMPs) derived from microbial cell-wall components. Insects and mammals conserve a signaling pathway of the innate immune system through cell-surface receptors called Tolls and Toll-like receptors (TLRs). Bacterial lipopolysaccharides (LPSs) are an important trigger of the horseshoe crab's innate immunity to infectious microorganisms. Horseshoe crabs' granular hemocytes respond specifically to LPS stimulation, inducing the secretion of various defense molecules from the granular hemocytes. Here, we show a cDNA which we named tToll, coding for a TLR identified from hemocytes of the horseshoe crab Tachypleus tridentatus. tToll is most closely related to Drosophila Toll in both domain architecture and overall length. Human TLRs have been suggested tc contain numerous PAMP-binding insertions located in the leucine-rich repeats (LRRs) of their ectodomains. However, the LRRs of tToll contained no obvious PAMP-binding insertions. Furthermore, tToll was non-specifically expressed in horseshoe crab tissues. These observations suggest that tToll does not function as an LPS receptor on granular hemocytes.

Original languageEnglish
Pages (from-to)106-115
Number of pages10
JournalImmunological Reviews
Volume198
DOIs
Publication statusPublished - Apr 1 2004

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Horseshoe Crabs
Toll-Like Receptors
Hemocytes
Leucine
Lipopolysaccharides
CD14 Antigens
Cell Surface Receptors
Cellular Structures
Innate Immunity
Cell Wall
Drosophila
Insects
Mammals
Immune System
Complementary DNA
Pathogen-Associated Molecular Pattern Molecules

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

A Toll-like receptor in horseshoe crabs. / Inamori, Kei Ichiro; Ariki, Shigeru; Kawabata, Shun-Ichiro.

In: Immunological Reviews, Vol. 198, 01.04.2004, p. 106-115.

Research output: Contribution to journalReview article

Inamori, Kei Ichiro ; Ariki, Shigeru ; Kawabata, Shun-Ichiro. / A Toll-like receptor in horseshoe crabs. In: Immunological Reviews. 2004 ; Vol. 198. pp. 106-115.
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