TY - JOUR
T1 - A Transcriptional Switch Point during Hematopoietic Stem and Progenitor Cell Ontogeny
AU - Sugiyama, Daisuke
AU - Joshi, Anagha
AU - Kulkeaw, Kasem
AU - Tan, Keai Sinn
AU - Yokoo-Inoue, Tomoko
AU - Mizuochi-Yanagi, Chiyo
AU - Yasuda, Kaori
AU - Doi, Atsushi
AU - Iino, Tadafumi
AU - Itoh, Masayoshi
AU - Nagao-Sato, Sayaka
AU - Tani, Kenzaburo
AU - Akashi, Koichi
AU - Hayashizaki, Yoshihide
AU - Suzuki, Harukazu
AU - Kawaji, Hideya
AU - Carninci, Piero
AU - Forrest, Alistair R.R.
N1 - Funding Information:
Acknowledgments The authors thank Miss. Yuka Tanaka and Yuka Horio for technical support, Dr. Elise Lamar for critical reading of the article, and the Ministry of Education, Culture, Sports, Science and Technology, the Ministry of Health, Labor and Welfare, and the Japan Society for the Promotion of Science for funding. K.S.T. is a recipient of a scholarship from the Tokyo Biochemical Research Foundation, Japan, and a MyPhD scholarship from the Ministry of Higher Education (MOHE), Malaysia. A.J. is a Chancellors Fellow at the University of Edinburgh.
Publisher Copyright:
© Mary Ann Liebert, Inc..
PY - 2017/3/1
Y1 - 2017/3/1
N2 - During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta (PL). Later, HSPCs expand in fetal liver (FL) and migrate to bone marrow (BM) shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. In this study, to assess transcriptional dynamics during development, we performed cap analysis of gene expression on 10 developmental murine HSPC populations isolated from the AGM region, PL, FL, and BM and identified 15,681 transcripts across HSPC ontogeny. We performed microarray analysis of AGM-derived HSPCs at 9.5 and 10.5 days postcoitum (dpc) and identified 40 differentially expressed genes, 23 confirmed as significantly changed by real-time polymerase chain reaction. We conclude that a transcriptional switch point occurs in HSPC ontogeny between 9.5 and 10.5 dpc in the AGM region.
AB - During mammalian embryogenesis, hematopoietic stem and progenitor cells (HSPCs) originate from mesoderm-derived endothelial cells in the aorta-gonad-mesonephros (AGM) region and placenta (PL). Later, HSPCs expand in fetal liver (FL) and migrate to bone marrow (BM) shortly before birth. Understanding global transcriptional regulation governing HSPC emergence from embryonic stem/induced pluripotent stem cells is necessary to devise clinical applications, such as novel transplantation approaches. In this study, to assess transcriptional dynamics during development, we performed cap analysis of gene expression on 10 developmental murine HSPC populations isolated from the AGM region, PL, FL, and BM and identified 15,681 transcripts across HSPC ontogeny. We performed microarray analysis of AGM-derived HSPCs at 9.5 and 10.5 days postcoitum (dpc) and identified 40 differentially expressed genes, 23 confirmed as significantly changed by real-time polymerase chain reaction. We conclude that a transcriptional switch point occurs in HSPC ontogeny between 9.5 and 10.5 dpc in the AGM region.
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U2 - 10.1089/scd.2016.0194
DO - 10.1089/scd.2016.0194
M3 - Article
C2 - 27848279
AN - SCOPUS:85013969126
SN - 1547-3287
VL - 26
SP - 314
EP - 327
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 5
ER -