A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant

Michio Doi, Ayako Shigenaga, Takashi Emi, Toshinori Kinoshita, Ken Ichiro Shimazaki

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.

Original languageEnglish
Pages (from-to)517-523
Number of pages7
JournalJournal of Experimental Botany
Volume55
Issue number396
DOIs
Publication statusPublished - Feb 1 2004

Fingerprint

blue light
Transgenes
Arabidopsis
transgenes
Phototropins
Light
mutants
receptors
Phototropism
Complementary DNA
stomatal movement
phototropism
leaf development
Chloroplasts
Plantae
chloroplasts

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant. / Doi, Michio; Shigenaga, Ayako; Emi, Takashi; Kinoshita, Toshinori; Shimazaki, Ken Ichiro.

In: Journal of Experimental Botany, Vol. 55, No. 396, 01.02.2004, p. 517-523.

Research output: Contribution to journalArticle

Doi, Michio ; Shigenaga, Ayako ; Emi, Takashi ; Kinoshita, Toshinori ; Shimazaki, Ken Ichiro. / A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant. In: Journal of Experimental Botany. 2004 ; Vol. 55, No. 396. pp. 517-523.
@article{d93adf697f824c1ab71230b00481ef75,
title = "A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant",
abstract = "Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.",
author = "Michio Doi and Ayako Shigenaga and Takashi Emi and Toshinori Kinoshita and Shimazaki, {Ken Ichiro}",
year = "2004",
month = "2",
day = "1",
doi = "10.1093/jxb/erh044",
language = "English",
volume = "55",
pages = "517--523",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "396",

}

TY - JOUR

T1 - A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1 phot2 double mutant

AU - Doi, Michio

AU - Shigenaga, Ayako

AU - Emi, Takashi

AU - Kinoshita, Toshinori

AU - Shimazaki, Ken Ichiro

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.

AB - Phototropins (phot1 and phot2) are suggested to be multifunctional blue-light (BL) receptors mediating phototropism, chloroplast movement, stomatal opening, and leaf expansion. The Arabidpsis phot1 phot2 double mutant lacks all of these responses. To confirm the requirement of phototropins in BL responses, the Arabidopsis phot1 phot2 double mutant was transformed with PHOT1 cDNA and the phenotypic restoration was analysed in the transformants. It was found that all BL responses were restored, although differentially, by the transformation of the Arabidopsis phot1 phot2 double mutant with PHOT1 cDNA. The results showed that phot1 was an essential component for all these BL responses in planta, and that the cellular level of phot1 might determine the individual BL responses.

UR - http://www.scopus.com/inward/record.url?scp=1142293853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1142293853&partnerID=8YFLogxK

U2 - 10.1093/jxb/erh044

DO - 10.1093/jxb/erh044

M3 - Article

C2 - 14739272

AN - SCOPUS:1142293853

VL - 55

SP - 517

EP - 523

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 396

ER -