TY - JOUR
T1 - A transgenic dwarf rat model as a tool for the study of calorie restriction and aging
AU - Yamaza, Haruyoshi
AU - Komatsu, Toshimitsu
AU - Chiba, Takuya
AU - Toyama, Hiroaki
AU - To, Kazuo
AU - Higami, Yoshikazu
AU - Shimokawa, Isao
N1 - Funding Information:
We thank Yutaka Araki and the staff in the laboratory animal center at Nagasaki University School of Medicine for their excellent technical support. We also thank Nippon Institute for Biological Science for providing the transgenic rat. This work was supported by the Research Grant for longevity Sciences (grants 11-C) from the Ministry of Health, Welfare, and Labor of Japan.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2004/2
Y1 - 2004/2
N2 - We have previously reported a long-lived transgenic dwarf rat model, in which the growth hormone (GH)-insulin like growth factor (IGF)-1 axis was selectively suppressed by overexpression of antisense GH transgene. Rats heterozygous for the transgene (tg/-) manifest phenotypes similar to those in calorie-restricted (CR) rats. To further characterize the transgenic rat in comparison with CR rats, the present study evaluated glucose and insulin tolerance in tg/- and control Wistar (-/-) rats at 6-9 months of age. Rats were fed ad libitum (AL) or 30% CR from 6 weeks of age. In CR rats, glucose disposal after glucose load was facilitated without any significant surge of serum insulin, and insulin tolerance test also indicated increased insulin sensitivity. In transgenic rats, similar findings were observed after glucose and insulin load, and CR in tg/- rats further facilitated glucose disposal during glucose and insulin tolerance tests. These findings suggest the presence of both common and separate mechanisms regulating the glucose-insulin system between CR and the reduced GH-IGF-1 axis paradigms. The transgenic rat model is, therefore, a useful one for studies of CR and aging.
AB - We have previously reported a long-lived transgenic dwarf rat model, in which the growth hormone (GH)-insulin like growth factor (IGF)-1 axis was selectively suppressed by overexpression of antisense GH transgene. Rats heterozygous for the transgene (tg/-) manifest phenotypes similar to those in calorie-restricted (CR) rats. To further characterize the transgenic rat in comparison with CR rats, the present study evaluated glucose and insulin tolerance in tg/- and control Wistar (-/-) rats at 6-9 months of age. Rats were fed ad libitum (AL) or 30% CR from 6 weeks of age. In CR rats, glucose disposal after glucose load was facilitated without any significant surge of serum insulin, and insulin tolerance test also indicated increased insulin sensitivity. In transgenic rats, similar findings were observed after glucose and insulin load, and CR in tg/- rats further facilitated glucose disposal during glucose and insulin tolerance tests. These findings suggest the presence of both common and separate mechanisms regulating the glucose-insulin system between CR and the reduced GH-IGF-1 axis paradigms. The transgenic rat model is, therefore, a useful one for studies of CR and aging.
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U2 - 10.1016/j.exger.2003.11.001
DO - 10.1016/j.exger.2003.11.001
M3 - Article
C2 - 15036422
AN - SCOPUS:0842283347
SN - 0531-5565
VL - 39
SP - 269
EP - 272
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 2
ER -