A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip

Hitoshi Hiura, Atsushi Sugawara, Hidehiko Ogawa, Rosalind M. John, Naoko Miyauchi, Yusuke Miyanari, Tokumasa Horiike, Yufeng Li, Nobuo Yaegashi, Hiroyuki Sasaki, Tomohiro Kono, Takahiro Arima

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The parent-of-origin specific expression of imprinted genes relies on DNA methylation of CpGdinucleotides at differentially methylated regions (DMRs) during gametogenesis. To date, four paternally methylated DMRs have been identified in screens based on conventional approaches. These DMRs are linked to the imprinted genes H19, Gtl2 (IG-DMR), Rasgrf1 and, most recently, Zdbf2 which encodes zinc finger, DBF-type containing 2. In this study, we applied a novel methylated-DNA immunoprecipitation-on-chip (meDIP-on-chip) method to genomic DNA from mouse parthenogenetic-and androgenetic-derived stem cells and sperm and identified 458 putative DMRs. This included the majority of known DMRs. We further characterized the paternally methylated Zdbf2/ ZDBF2 DMR. In mice, this extensive germ line DMR spanned 16 kb and possessed an unusual tripartite structure. Methylation was dependent on DNA methyltransferase 3a (Dnmt3a), similar to H19 DMR and IG-DMR. In both humans and mice, the adjacent gene, Gpr1/GPR1, which encodes a G-proteincoupled receptor 1 protein with transmembrane domain, was also imprinted and paternally expressed. The Gpr1-Zdbf2 domain was most similar to the Rasgrf1 domain as both DNA methylation and the actively expressed allele were in cis on the paternal chromosome. This work demonstrates the effectiveness of meDIP-on-chip as a technique for identifying DMRs.

Original languageEnglish
Pages (from-to)4929-4945
Number of pages17
JournalNucleic acids research
Volume38
Issue number15
DOIs
Publication statusPublished - Apr 29 2010
Externally publishedYes

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ras-GRF1
Chromosomes, Human, Pair 1
Immunoprecipitation
DNA Methylation
DNA
Gametogenesis
Zinc Fingers
Germ Cells
Methylation
Genes
Spermatozoa
Stem Cells
Chromosomes
Alleles
Gene Expression
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip. / Hiura, Hitoshi; Sugawara, Atsushi; Ogawa, Hidehiko; John, Rosalind M.; Miyauchi, Naoko; Miyanari, Yusuke; Horiike, Tokumasa; Li, Yufeng; Yaegashi, Nobuo; Sasaki, Hiroyuki; Kono, Tomohiro; Arima, Takahiro.

In: Nucleic acids research, Vol. 38, No. 15, 29.04.2010, p. 4929-4945.

Research output: Contribution to journalArticle

Hiura, H, Sugawara, A, Ogawa, H, John, RM, Miyauchi, N, Miyanari, Y, Horiike, T, Li, Y, Yaegashi, N, Sasaki, H, Kono, T & Arima, T 2010, 'A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip', Nucleic acids research, vol. 38, no. 15, pp. 4929-4945. https://doi.org/10.1093/nar/gkq200
Hiura, Hitoshi ; Sugawara, Atsushi ; Ogawa, Hidehiko ; John, Rosalind M. ; Miyauchi, Naoko ; Miyanari, Yusuke ; Horiike, Tokumasa ; Li, Yufeng ; Yaegashi, Nobuo ; Sasaki, Hiroyuki ; Kono, Tomohiro ; Arima, Takahiro. / A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip. In: Nucleic acids research. 2010 ; Vol. 38, No. 15. pp. 4929-4945.
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AU - Horiike, Tokumasa

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