A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans

Yu Hayashi, Takaaki Hirotsu, Ryo Iwata, Eriko Kage-Nakadai, Hirofumi Kunitomo, Takeshi Ishihara, Yuichi Iino, Takeo Kubo

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Abstract

The molecular mechanism by which neurites are selected for elimination or incorporation into the mature circuit during developmental pruning remains unknown. The trophic theory postulates that local cues provided by target or surrounding cells act to inhibit neurite elimination. However, no widely conserved factor mediating this trophic function has been identified. We found that the developmental survival of specific neurites in Caenorhabditis elegans largely depends on detection of the morphogen Wnt by the Ror kinase CAM-1, which is a transmembrane tyrosine kinase with a Frizzled domain. Mutations in Wnt genes or in cam-1 enhanced neurite elimination, whereas overexpression of cam-1 inhibited neurite elimination in a Wnt-dependent manner. Moreover, mutations in these genes counteracted the effect of a mutation in mbr-1, which encodes a transcription factor that promotes neurite elimination. These results reveal the trophic role of an atypical Wnt pathway and reinforce the classical model of developmental pruning.

Original languageEnglish
Pages (from-to)981-987
Number of pages7
JournalNature Neuroscience
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 1 2009

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Hayashi, Y., Hirotsu, T., Iwata, R., Kage-Nakadai, E., Kunitomo, H., Ishihara, T., ... Kubo, T. (2009). A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans. Nature Neuroscience, 12(8), 981-987. https://doi.org/10.1038/nn.2347