A two-piece derivative of a group i intron RNA as a platform for designing self-assembling RNA templates to promote peptide ligation

Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa

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    1 Citation (Scopus)

    Abstract

    Multicomponent RNA-peptide complexes are attractive from the viewpoint of artificial design of functional biomacromolecular systems. We have developed self-folding and self-assembling RNAs that serve as templates to assist chemical ligation between two reactive peptides with RNA-binding capabilities. The design principle of previous templates, however, can be applied only to limited classes of RNA-binding peptides. In this study, we employed a two-piece derivative of a group I intron RNA from the Tetrahymena large subunit ribosomal RNA (LSU rRNA) as a platform for new template RNAs. In this group I intron-based self-assembling platform, modules for the recognition of substrate peptides can be installed independently from modules holding the platform structure. The new self-assembling platform allows us to expand the repertoire of substrate peptides in template RNA design.

    Original languageEnglish
    Article number305867
    JournalJournal of Nucleic Acids
    Volume2012
    DOIs
    Publication statusPublished - 2012

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology

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